ArticlePloS one2011
GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 165 papers.
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Who cites it
165 citing papers in PubMed.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- Distinct thresholds condition sense posttranscriptional gene silencing initiation and amplification.The Plant cell · 2026Article
- The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use inACS synthetic biology · 2026Article
- Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants.Plant biotechnology journal · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Introduction of the Ribo-BiFC method to plants using a split mVenus approach.Plant methods · 2026Article
- Dual-Luciferase Assay System for the Quantification of Promoter Activity.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Functional diversity of sunflower DGAT1 and DGAT2 enzymes underlying seed triacylglycerol biosynthesis.Frontiers in plant science · 2026Article
- Golden Gate Assembly for Genome Editing in Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- Ipecac alkaloid biosynthesis in two evolutionarily distant plants.Nature chemical biology · 2025Article
- A combination of cry (Cry2Ab) and plant lectin (PTA) genes confers resistance against major sucking and chewing insect pests of cotton.Molecular biology reports · 2025Article
- Scaling DNA engineering.Trends in biotechnology · 2025Review
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- DASH: a versatile and high-capacity gene stacking system for plant synthetic biology.Plant biotechnology journal · 2025Review
- CRISPR-GATE: a one-stop repository and guide to computational resources for genome editing experimentation.Briefings in bioinformatics · 2025Review
- Synthesis of large single-transcript pathways from oligonucleotide pools: Design of STARBURST, an autobioluminescent reporter.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Gibberellin-deactivating GA2OX enzymes act as a hub for auxin-gibberellin cross talk inProceedings of the National Academy of Sciences of the United States of America · 2025Article
105 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of standard DNA parts and to combine them binarily to build increasingly complex multigene constructs. The relative position of type IIS restriction sites inside pDGB vectors introduces a double loop ("braid") topology in the cloning strategy that allows the indefinite growth of composite parts through the succession of iterative assembling steps, while the overall simplicity of the system is maintained. We propose the use of GoldenBraid as an assembly standard for Plant Synthetic Biology. For this purpose we have GB-adapted a set of binary plasmids for A. tumefaciens-mediated plant transformation. Fast GB-engineering of several multigene T-DNAs, including two alternative modules made of five reusable devices each, and comprising a total of 19 basic parts are also described.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.