ArticleCancer biology & therapy2011
Frequent detection of infectious xenotropic murine leukemia virus (XMLV) in human cultures established from mouse xenografts.
Article in Cancer biology & therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Presence of complete murine viral genome sequences in patient-derived xenografts.Nature communications · 2021Article
- Characterization of a new case of XMLV (Bxv1) contamination in the human cell line Hep2 (clone 2B).Scientific reports · 2020Article
- Murine Endogenous Retroviruses Are Detectable in Patient-Derived Xenografts but Not in Patient-Individual Cell Lines of Human Colorectal Cancer.Frontiers in microbiology · 2018Article
- Recombinant Origins of Pathogenic and Nonpathogenic Mouse Gammaretroviruses with Polytropic Host Range.Journal of virology · 2017Article
- Sequence Diversity, Intersubgroup Relationships, and Origins of the Mouse Leukemia Gammaretroviruses of Laboratory and Wild Mice.Journal of virology · 2016Article
- From Mice to Men and Back: An Assessment of Preclinical Model Systems for the Study of Lung Cancers.Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer · 2016Review
- Xenotropic retrovirus Bxv1 in human pancreatic β cell lines.The Journal of clinical investigation · 2016Article
- Frequent infection of human cancer xenografts with murine endogenous retroviruses in vivo.Viruses · 2015Article
- Prevalence and characterization of murine leukemia virus contamination in human cell lines.PloS one · 2015Article
- The saga of XMRV: a virus that infects human cells but is not a human virus.Emerging microbes & infections · 2014Review
- Generation of multiple replication-competent retroviruses through recombination between PreXMRV-1 and PreXMRV-2.Journal of virology · 2013Article
- The clinical relevance of cancer cell lines.Journal of the National Cancer Institute · 2013Article
- No association between XMRV or related gammaretroviruses in Australian prostate cancer patients.Virology journal · 2013Article
- Infection of Xenotransplanted Human Cell Lines by Murine Retroviruses: A Lesson Brought Back to Light by XMRV.Frontiers in oncology · 2013Article
- Sensitivity of PCR assays for murine gammaretroviruses and mouse contamination in human blood samples.PloS one · 2012Article
- Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.PloS one · 2012Article
- Multiple sources of contamination in samples from patients reported to have XMRV infection.PloS one · 2012Article
- Characterization, mapping, and distribution of the two XMRV parental proviruses.Journal of virology · 2012Article
- The human lung adenocarcinoma cell line EKVX produces an infectious xenotropic murine leukemia virus.Viruses · 2011Article
- Failure to confirm XMRV/MLVs in the blood of patients with chronic fatigue syndrome: a multi-laboratory study.Science (New York, N.Y.) · 2011Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeTo investigate the frequency of xenotropic murine leukemia virus (MLV) presence in human cell lines established from mouse xenografts and to search for the evidence of horizontal viral spread to other cell lines.
resultsSix of 23 (26%) mouse DNA free xenograft cultures were strongly positive for MLV and their sequences had greater than 99% homology to known MLV strains. Four of five available supernatant fluids from these viral positive cultures were strongly positive for RT activity. Three of these supernatant fluids were studied to confirm the infectivity of the released virions for other human culture cells. Of the 78 non-xenograft derived cell lines maintained in the xenograft culture-containing facilities, 13 (17%) were positive for MLV, including XMRV, a virus strain first identified in human tissues. By contrast, all 50 cultures maintained in a xenograft culture-free facility were negative for viral sequences. METHODOLOGY: We examined xenograft tumor cell lines from seven independent laboratories and 128 non-xenografted tumor cell lines. Cell line DNA was examined for mouse DNA contamination, and by 3 Taqman qPCR assays targeting the gag, env or pol regions of MLV. Sequencing was used for viral strain identification. Supernatant fluids were tested for reverse transcriptase (RT) activity.
conclusionsHuman cultures derived after mouse xenografting frequently contain and release highly infectious xenotropic MLV viruses. Laboratories working with xenograft-derived human cultures should be aware of the risk of contamination with potentially biohazardous human-tropic mouse viruses and their horizontal spread to other cultures.
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