ArticleAmerican journal of physiology. Endocrinology and metabolism2011
Homocysteine suppresses lipolysis in adipocytes by activating the AMPK pathway.
Article in American journal of physiology. Endocrinology and metabolism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Effect of Betaine on Reducing Body Fat-A Systematic Review and Meta-Analysis of Randomized Controlled Trials.Nutrients · 2019Pooled it
- Trial
- Homocysteine is a risk factor for reduced ejection fraction in children with myocarditis: a single-center study.Frontiers in pediatrics · 2026Article
- Therapeutic role and mechanisms of vitamins for controlling obesity/adiposity-a brief overview of recent studies.International journal of obesity (2005) · 2026Review
- Uncovering Hyperhomocysteinemia: Global Risk Patterns and Molecular Disruption in Brain and Vascular Health.Journal of neurochemistry · 2025Review
- Evaluation of Total Homocysteine Levels in Relation to Abdominal Fat Mass and Traditional Cardiovascular Risk Factors in Overweight and Obese Adolescents.Life (Basel, Switzerland) · 2025Article
- Bioelectric medicine: unveiling the therapeutic potential of micro-current stimulation.Biomedical engineering letters · 2024Review
- Nicotinamide N-methyltransferase (NNMT): a novel therapeutic target for metabolic syndrome.Frontiers in pharmacology · 2024Review
- Association between Variation in Body Fat Mass Magnitude and Intake of Nutrients, including Carbohydrates, Fat, and B Vitamins, in a Cohort of Highly Trained Female Athletes.Foods (Basel, Switzerland) · 2023Article
- Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice.Nutrients · 2023Article
- Article
- Causal effects of B vitamins and homocysteine on obesity and musculoskeletal diseases: A Mendelian randomization study.Frontiers in nutrition · 2022Article
- Nuclear factor erythroid 2-related factor 2 activation mediates hyperhomocysteinemia-associated lipolysis suppression in adipocytes.Experimental biology and medicine (Maywood, N.J.) · 2018Article
- Homocysteine regulates fatty acid and lipid metabolism in yeast.The Journal of biological chemistry · 2018Article
- Schisandrin B regulates lipid metabolism in subcutaneous adipocytes.Scientific reports · 2017Article
- Comparative transcriptomic analysis of mice liver treated with different AMPK activators in a mice model of atherosclerosis.Oncotarget · 2017Article
- Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry.Analytical and bioanalytical chemistry · 2017Article
- An AMP-activated protein kinase-stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice.Nature medicine · 2016Article
- Effects of homocysteine on adipocyte differentiation and CD36 gene expression in 3T3-L1 adipocytes.Journal of cell communication and signaling · 2016Article
- Multiplexed microfluidic enzyme assays for simultaneous detection of lipolysis products from adipocytes.Analytical and bioanalytical chemistry · 2014Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Hyperhomocysteinemia (HHcy) is an independent risk factor for coronary artery disease. Emerging evidence suggests that HHcy is also associated with adipocyte tissue dysfunction. One of the principal functions of adipose tissue is to provide energy substrate via lipolysis. In the present study, we investigated the effects of homocysteine (Hcy) on lipolysis in adipocytes. We found that Hcy inhibited release of glycerol and fatty acids, two typical indicators of the lipolytic response, in primary adipocytes and fully differentiated 3T3-L1 adipocytes in a dose-dependent manner under both basal and isoproterenol-stimulated conditions. In differentiated 3T3-L1 adipocytes, decreased glycerol and free fatty acid (FFA) release was associated with elevation of intracellular TG content. Further studies showed that Hcy-mediated antilipolytic responses were independent of the cyclic AMP-PKA and MEK-ERK1/2 pathways. However, Hcy increased phosphorylation levels of AMP-activated protein kinase (AMPK) and its downstream enzyme acetyl-CoA carboxylase. Compound C, an AMPK inhibitor, abolished Hcy-induced reduction of glycerol and FFA release under both basal and isoproterenol-stimulated conditions. Furthermore, AMPKα1 siRNA reversed Hcy-inhibited glycerol release. Supplementation of exogenous Hcy in the diet for 2 wk lowered circulating glycerol and FFA levels. Moreover, Hcy supplementation was associated with elevated leptin levels and reduced adiponectin levels in plasma. These results show that Hcy inhibits lipolysis through a pathway that involves AMPK activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.