ArticleNucleic acids research2011
SUS1 introns are required for efficient mRNA nuclear export in yeast.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Unraveling gene expression: a beginner's guide from chromatin modifications to mRNA export inNucleus (Austin, Tex.) · 2025Review
- Biochemical and genetic evidence supports Fyv6 as a second-step splicing factor inRNA (New York, N.Y.) · 2023Article
- Intron retention decreases METTL3 expression by inhibiting mRNA export to the cytoplasm.BMB reports · 2023Article
- Sus1 maintains a normal lifespan through regulation of TREX-2 complex-mediated mRNA export.Aging · 2022Article
- Article
- An insight into structural plasticity and conformational transitions of transcriptional co-activator Sus1.PloS one · 2020Article
- Intron Retention as a Mode for RNA-Seq Data Analysis.Frontiers in genetics · 2020Review
- Intron retention enhances gene regulatory complexity in vertebrates.Genome biology · 2017Article
- Posttranscriptional Regulation of Gcr1 Expression and Activity Is Crucial for Metabolic Adjustment in Response to Glucose Availability.Molecular cell · 2016Article
- Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening.PLoS genetics · 2016Article
- An intronic RNA structure modulates expression of the mRNA biogenesis factor Sus1.RNA (New York, N.Y.) · 2016Article
- Two Routes to Genetic Suppression of RNA Trimethylguanosine Cap Deficiency via C-Terminal Truncation of U1 snRNP Subunit Snp1 or Overexpression of RNA Polymerase Subunit Rpo26.G3 (Bethesda, Md.) · 2015Article
- Quantification of pre-mRNA escape rate and synergy in splicing.Nucleic acids research · 2014Article
- Review
- Introns regulate gene expression in Cryptococcus neoformans in a Pab2p dependent pathway.PLoS genetics · 2013Article
- Regulated pre-mRNA splicing: the ghostwriter of the eukaryotic genome.Biochimica et biophysica acta · 2012Review
- Alternative splicing regulates targeting of malate dehydrogenase in Yarrowia lipolytica.DNA research : an international journal for rapid publication of reports on genes and genomes · 2012Article
- Key features of the two-intron Saccharomyces cerevisiae gene SUS1 contribute to its alternative splicing.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient coupling between mRNA synthesis and export is essential for gene expression. Sus1/ENY2, a component of the SAGA and TREX-2 complexes, is involved in both transcription and mRNA export. While most yeast genes lack introns, we previously reported that yeast SUS1 bears two. Here we show that this feature is evolutionarily conserved and critical for Sus1 function. We determine that while SUS1 splicing is inefficient, it responds to cellular conditions, and intronic mutations either promoting or blocking splicing lead to defects in mRNA export and cell growth. Consistent with this, we find that an intron-less SUS1 only partially rescues sus1Δ phenotypes. Remarkably, splicing of each SUS1 intron is also affected by the presence of the other and by SUS1 exonic sequences. Moreover, by following SUS1 RNA and protein levels we establish that nonsense-mediated decay (NMD) pathway and the splicing factor Mud2 both play a role in SUS1 expression. Our data (and those of the accompanying work by Hossain et al.) provide evidence of the involvement of splicing, translation, and decay in the regulation of early events in mRNP biogenesis; and imply the additional requirement for a balance in splicing isoforms from a single gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.