ArticleNucleic acids research2011
Key features of the two-intron Saccharomyces cerevisiae gene SUS1 contribute to its alternative splicing.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Unraveling gene expression: a beginner's guide from chromatin modifications to mRNA export inNucleus (Austin, Tex.) · 2025Review
- Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo.Nature structural & molecular biology · 2025Article
- Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing inInternational journal of molecular sciences · 2025Article
- Transcriptome analysis of two isolates of the tomato pathogen Cladosporium fulvum, uncovers genome-wide patterns of alternative splicing during a host infection cycle.PLoS pathogens · 2024Article
- Replicative aging in yeast involves dynamic intron retention patterns associated with mRNA processing/export and protein ubiquitination.Microbial cell (Graz, Austria) · 2024Article
- Biochemical and genetic evidence supports Fyv6 as a second-step splicing factor inRNA (New York, N.Y.) · 2023Article
- Phylogenomics of aiScience · 2022Article
- Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi.Biotechnology for biofuels and bioproducts · 2022Article
- A broad analysis of splicing regulation in yeast using a large library of synthetic introns.PLoS genetics · 2021Article
- Translation Initiation Site Profiling Reveals Widespread Synthesis of Non-AUG-Initiated Protein Isoforms in Yeast.Cell systems · 2020Article
- Intron specificity in pre-mRNA splicing.Current genetics · 2018Review
- Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast.Genome research · 2018Article
- Chromatin-remodeling SWI/SNF complex regulates coenzyme QThe Journal of biological chemistry · 2017Article
- mRNA detection in budding yeast with single fluorophores.Nucleic acids research · 2017Article
- The histone variant H2A.Z promotes efficient cotranscriptional splicing inGenes & development · 2017Article
- Circular RNAs: A Novel Player in Development and Disease of the Central Nervous System.Frontiers in cellular neuroscience · 2017Review
- Selection on Position of Nonsense Codons in Introns.Genetics · 2016Article
- Intron retention-dependent gene regulation in Cryptococcus neoformans.Scientific reports · 2016Article
- Posttranscriptional Regulation of Gcr1 Expression and Activity Is Crucial for Metabolic Adjustment in Response to Glucose Availability.Molecular cell · 2016Article
- An intronic RNA structure modulates expression of the mRNA biogenesis factor Sus1.RNA (New York, N.Y.) · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Alternative pre-mRNA splicing allows dramatic expansion of the eukaryotic proteome and facilitates cellular response to changes in environmental conditions. The Saccharomyces cerevisiae gene SUS1, which encodes a protein involved in mRNA export and histone H2B deubiquitination, contains two introns; non-canonical sequences in the first intron contribute to its retention, a common form of alternative splicing in plants and fungi. Here we show that the pattern of SUS1 splicing changes in response to environmental change such as temperature elevation, and the retained intron product is subject to nonsense-mediated decay. The activities of different splicing factors determine the pattern of SUS1 splicing, including intron retention and exon skipping. Unexpectedly, removal of the 3' intron is affected by splicing of the upstream intron, suggesting that cross-exon interactions influence intron removal. Production of different SUS1 isoforms is important for cellular function, as we find that the temperature sensitivity and histone H2B deubiquitination defects observed in sus1Δ cells are only partially suppressed by SUS1 cDNA, but SUS1 that is able to undergo splicing complements these phenotypes. These data illustrate a role for S. cerevisiae alternative splicing in histone modification and cellular function and reveal important mechanisms for splicing of yeast genes containing multiple introns.
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