ArticleThe Biochemical journal2011
The role of the protein kinase A pathway in the response to alkaline pH stress in yeast.
Article in The Biochemical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Transcriptional Responses to Alkaline pH Across Fungi: Common and Differential Features, and Biotechnological Applications.International journal of molecular sciences · 2025Review
- Ppn2 Polyphosphatase Improves the Ability ofJournal of fungi (Basel, Switzerland) · 2024Article
- Unveiling genetic anchors in saccharomyces cerevisiae: QTL mapping identifies IRA2 as a key player in ethanol tolerance and beyond.Molecular genetics and genomics : MGG · 2024Article
- Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.Bioscience reports · 2024Article
- Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii.Microbial cell factories · 2024Article
- Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization.Microbial cell factories · 2023Article
- TheInternational journal of molecular sciences · 2023Article
- Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistantFrontiers in microbiology · 2023Article
- Assessment of Yeasts as Potential Probiotics: A Review of Gastrointestinal Tract Conditions and Investigation Methods.Journal of fungi (Basel, Switzerland) · 2022Review
- Genome-Wide Identification of Cellular Pathways and Key Genes That Respond to Sodium Bicarbonate Stress inFrontiers in microbiology · 2022Article
- Intrinsically disordered signaling proteins: Essential hub players in the control of stress responses in Saccharomyces cerevisiae.PloS one · 2022Article
- Extracellular pH and high concentration of potassium regulate the primary necrosis in the yeast Saccharomyces cerevisiae.Archives of microbiology · 2021Article
- Isolation and identification of carotenoid-producingBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Screening and Genetic Network Analysis of Genes Involved in Freezing and Thawing Resistance inGenes · 2021Article
- Simultaneous Production of Lipids and Carotenoids by the Red Yeast Rhodotorula from Waste Glycerol Fraction and Potato Wastewater.Applied biochemistry and biotechnology · 2019Article
- Article
- pH homeostasis links the nutrient sensing PKA/TORC1/Sch9 ménage-à-trois to stress tolerance and longevity.Microbial cell (Graz, Austria) · 2018Review
- A novel connection between the Cell Wall Integrity and the PKA pathways regulates cell wall stress response in yeast.Scientific reports · 2017Article
- Regulation of the reserve carbohydrate metabolism by alkaline pH and calcium in Neurospora crassa reveals a possible cross-regulation of both signaling pathways.BMC genomics · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exposure of Saccharomyces cerevisiae to alkaline pH provokes a stress condition that generates a compensatory reaction. In the present study we examined a possible role for the PKA (protein kinase A) pathway in this response. Phenotypic analysis revealed that mutations that activate the PKA pathway (ira1 ira2, bcy1) tend to cause sensitivity to alkaline pH, whereas its deactivation enhances tolerance to this stress. We observed that alkalinization causes a transient decrease in cAMP, the main regulator of the pathway. Alkaline pH causes rapid nuclear localization of the PKA-regulated Msn2 transcription factor which, together with Msn4, mediates a general stress response by binding with STRE (stress response element) sequences in many promoters. Consequently, a synthetic STRE-LacZ reporter shows a rapid induction in response to alkaline stress. A msn2 msn4 mutant is sensitive to alkaline pH, and transcriptomic analysis reveals that after 10 min of alkaline stress, the expression of many induced genes (47%) depends, at least in part, on the presence of Msn2 and Msn4. Taken together, these results demonstrate that inhibition of the PKA pathway by alkaline pH represents a substantial part of the adaptive response to this kind of stress and that this response involves Msn2/Msn4-mediated genome expression remodelling. However, the relevance of attenuation of PKA in high pH tolerance is probably not restricted to regulation of Msn2 function.
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