ArticleMethods in molecular biology (Clifton, N.J.)2011
Assessment of development and chemotaxis in Dictyostelium discoideum mutants.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- A negative feedback loop between small GTPase Rap1 and mammalian tumor suppressor homologue KrsB regulates cell-substrate adhesion inMolecular biology of the cell · 2025Article
- Optogenetic control of cAMP oscillations reveals frequency-selective transcription factor dynamics in Dictyostelium.Development (Cambridge, England) · 2025Article
- Trade-offs in concentration sensing in dynamic environments.Biophysical journal · 2024Article
- Current methods for studying metastatic potential of tumor cells.Cancer cell international · 2022Review
- Differential Roles of Actin Crosslinking Proteins Filamin and α-Actinin in Shear Flow-Induced Migration ofFrontiers in cell and developmental biology · 2021Article
- Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation.Journal of visualized experiments : JoVE · 2017Article
- Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Chemical and mechanical stimuli act on common signal transduction and cytoskeletal networks.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A set of genes conserved in sequence and expression traces back the establishment of multicellularity in social amoebae.BMC genomics · 2016Article
- A deep coverage Dictyostelium discoideum genomic DNA library replicates stably in Escherichia coli.Genomics · 2015Article
- Novel protein Callipygian defines the back of migrating cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum.Science signaling · 2015Article
- How to understand and outwit adaptation.Developmental cell · 2014Article
- Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer.Science (New York, N.Y.) · 2014Article
- Different CHD chromatin remodelers are required for expression of distinct gene sets and specific stages during development of Dictyostelium discoideum.Development (Cambridge, England) · 2013Article
- Lipid composition of multilamellar bodies secreted by Dictyostelium discoideum reveals their amoebal origin.Eukaryotic cell · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Studies using the social amoeba Dictyostelium discoideum have greatly contributed to the current understanding of the signaling network that underlies chemotaxis. Since directed migration is essential for normal D. discoideum multicellular development, mutants with chemotactic impairments are likely to have abnormal developmental morphologies. We have used multicellular development as a readout in a screen of mutants to identify new potential regulators of chemotaxis. In this chapter, we describe how mutants generated by restriction enzyme-mediated integration (REMI) are analyzed, from assessment of development to detailed characterization of 3',5'-cyclic adenosine monophosphate (cAMP)-induced responses. Two complementary approaches, plating cells either clonally on a bacterial lawn or as a population on non-nutrient agar, are used to evaluate multicellular development. Once mutants with aberrant developmental phenotypes are identified, their chemotaxis toward cAMP is assessed by both small population and micropipette assays. Furthermore, mutants are tested for defects in both general and specific signaling pathways by examining the recruitment of actin-binding LimE(Δcoil) or PIP3-binding PH domains to the plasma membrane in response to cAMP stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.