ArticleJournal of bacteriology1990
Cloning and cell cycle-dependent expression of DNA replication gene dnaC from Caulobacter crescentus.
Article in Journal of bacteriology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts with and stabilizes the histone acetyltransferase GW6a to control the grain size in rice.The Plant cell · 2021Article
- Auxin biosynthesis by the YUCCA genes in rice.Plant physiology · 2007Article
- A temperature-sensitive mutation in the dnaE gene of Caulobacter crescentus that prevents initiation of DNA replication but not ongoing elongation of DNA.Journal of bacteriology · 2004Article
- Replication intermediate analysis confirms that chromosomal replication origin initiates from an unusual intergenic region in Caulobacter crescentus.Nucleic acids research · 2001Article
- CtrA mediates a DNA replication checkpoint that prevents cell division in Caulobacter crescentus.The EMBO journal · 2000Article
- Cell cycle expression and transcriptional regulation of DNA topoisomerase IV genes in caulobacter.Journal of bacteriology · 1999Article
- Genetic analysis of mecillinam-resistant mutants of Caulobacter crescentus deficient in stalk biosynthesis.Journal of bacteriology · 1998Article
- An essential, multicomponent signal transduction pathway required for cell cycle regulation in Caulobacter.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Translation of the leaderless Caulobacter dnaX mRNA.Journal of bacteriology · 1997Article
- An alkB gene homolog is differentially transcribed during the Caulobacter crescentus cell cycle.Journal of bacteriology · 1997Article
- Identification, characterization, and chromosomal organization of cell division cycle genes in Caulobacter crescentus.Journal of bacteriology · 1997Article
- Posttranscriptional regulation of Caulobacter flagellin genes by a late flagellum assembly checkpoint.Journal of bacteriology · 1997Article
- Transcription of genes encoding DNA replication proteins is coincident with cell cycle control of DNA replication in Caulobacter crescentus.Journal of bacteriology · 1997Article
- Identification of a novel response regulator required for the swarmer-to-stalked-cell transition in Caulobacter crescentus.Journal of bacteriology · 1995Article
- An essential single domain response regulator required for normal cell division and differentiation in Caulobacter crescentus.The EMBO journal · 1995Article
- Coordinate cell cycle control of a Caulobacter DNA methyltransferase and the flagellar genetic hierarchy.Journal of bacteriology · 1995Article
- Regulation of cellular differentiation in Caulobacter crescentus.Microbiological reviews · 1995Review
- Multiple structural proteins are required for both transcriptional activation and negative autoregulation of Caulobacter crescentus flagellar genes.Journal of bacteriology · 1994Article
- Expression of Caulobacter dnaA as a function of the cell cycle.Journal of bacteriology · 1994Article
- Asymmetric expression of the gyrase B gene from the replication-competent chromosome in the Caulobacter crescentus predivisional cell.Journal of bacteriology · 1993Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome replication in the asymmetrically dividing bacteria Caulobacter crescentus is discontinuous with the new, motile swarmer cell undergoing an obligatory presynthetic gap period (G1 period) of 60 min before the initiation of DNA synthesis and stalk formation. To examine the regulation of the cell division cycle at the molecular level, we have cloned the DNA chain elongation gene dnaC from a genomic DNA library constructed in cosmid vector pLAFR1-7. To ensure that the cloned sequence corresponded to dnaC, we isolated the gene by genetic complementation of the temperature-sensitive allele dnaC303 on DNA fragment that contained a Tn5 insertion element tightly linked by transduction to dnaC. The size of the dnaC gene was estimated to be 1,500 bp or less based on the pattern of complementation by subcloned restriction and BAL 31 deletion fragments. Nuclease S1 assays were used to map the transcription start site and to determine the pattern of dnaC expression in the cell cycle. Large amounts of the dnaC transcript began to accumulate only in the late G1 period of the swarmer cell and then peaked early during chromosome replication. We confirmed that the gene is periodically transcribed by monitoring the rate of beta-galactosidase synthesis directed by a dnaC promoter-lacZ fusion in a synchronous cell culture. dnaC is the first C. crescentus cell cycle gene whose regulation has been reported, and the discontinuous pattern of its expression suggests that the DNA synthetic period in these dimorphic bacteria is regulated in part by the stage-specific expression of DNA replication genes.
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