ArticlePloS one2011
Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucata.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 119 citations in OpenAlex.
- Ocean acidification disrupts the biomineralization process in the oyster Crassostrea virginica via intracellular calcium signaling dysregulation.Communications biology · 2026Article
- Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches.Marine biotechnology (New York, N.Y.) · 2025Article
- The Gln-rich protein twip1 promotes high-density {110} twins formation in the shell of the limpetProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring proteins within the coccolith matrix.Scientific reports · 2024Article
- RNA Interference Applied to Crustacean Aquaculture.Biomolecules · 2024Review
- Transcriptomic analysis of shell repair and biomineralization in the blue mussel, Mytilus edulis.BMC genomics · 2021Article
- Mobilizing molluscan models and genomes in biology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation.Marine biotechnology (New York, N.Y.) · 2021Article
- Hyperaccumulation of cadmium by scallop Chlamys farreri revealed by comparative transcriptome analysis.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2020Article
- A shell matrix protein of Pinctada mazatlanica produces nacre platelets in vitro.Scientific reports · 2020Article
- PfSMAD1/5 Can Interact with PfSMAD4 to Inhibit PfMSX to Regulate Shell Biomineralization in Pinctada fucata martensii.Marine biotechnology (New York, N.Y.) · 2020Article
- Histological and Expression Differences Among Different Mantle Regions of the Yesso Scallop (Patinopecten yessoensis) Provide Insights into the Molecular Mechanisms of Biomineralization and Pigmentation.Marine biotechnology (New York, N.Y.) · 2019Article
- Glycan Binding Profiling of Jacalin-Related Lectins from theInternational journal of molecular sciences · 2019Article
- Identification and Characterization of microRNAs and Their Predicted Functions in Biomineralization in the Pearl Oyster (Biology · 2019Article
- Expression of calcification-related ion transporters during blue mussel larval development.Ecology and evolution · 2019Article
- Composite Materials Design: Biomineralization Proteins and the Guided Assembly and Organization of Biomineral Nanoparticles.Materials (Basel, Switzerland) · 2019Review
- The evolution of molluscs.Biological reviews of the Cambridge Philosophical Society · 2019Article
- Piwi-interacting RNA (piRNA) expression patterns in pearl oyster (Pinctada fucata) somatic tissues.Scientific reports · 2019Article
- Mantle transcriptome sequencing ofPeerJ · 2019Article
- Transcriptomic responses to predator kairomones in embryos of the aquatic snailEcology and evolution · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mollusk shell formation is a fascinating aspect of biomineralization research. Shell matrix proteins play crucial roles in the control of calcium carbonate crystallization during shell formation in the pearl oyster, Pinctada fucata. Characterization of biomineralization-related genes during larval development could enhance our understanding of shell formation. Genes involved in shell biomineralization were isolated by constructing three suppression subtractive hybridization (SSH) libraries that represented genes expressed at key points during larval shell formation. A total of 2,923 ESTs from these libraries were sequenced and gave 990 unigenes. Unigenes coding for secreted proteins and proteins with tandem-arranged repeat units were screened in the three SSH libraries. A set of sequences coding for genes involved in shell formation was obtained. RT-PCR and in situ hybridization assays were carried out on five genes to investigate their spatial expression in several tissues, especially the mantle tissue. They all showed a different expression pattern from known biomineralization-related genes. Inhibition of the five genes by RNA interference resulted in different defects of the nacreous layer, indicating that they all were involved in aragonite crystallization. Intriguingly, one gene (UD_Cluster94.seq.Singlet1) was restricted to the 'aragonitic line'. The current data has yielded for the first time, to our knowledge, a suite of biomineralization-related genes active during the developmental stages of P. fucata, five of which were responsible for nacreous layer formation. This provides a useful starting point for isolating new genes involved in shell formation. The effects of genes on the formation of the 'aragonitic line', and other areas of the nacreous layer, suggests a different control mechanism for aragonite crystallization initiation from that of mature aragonite growth.
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