ArticlePloS one2011
Fatal outcome in bacteremia is characterized by high plasma cell free DNA concentration and apoptotic DNA fragmentation: a prospective cohort study.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Role of cell-free DNA levels in the diagnosis and prognosis of sepsis and bacteremia: A systematic review and meta-analysis.PloS one · 2024Pooled it
- Plasma concentration of parasite DNA as a measure of disease severity in falciparum malaria.The Journal of infectious diseases · 2015Trial
- Plasma levels of mitochondrial DNA in patients presenting to the emergency department with sepsis.Shock (Augusta, Ga.) · 2012Trial
- Plasma circulating free DNA in chronic schizophrenia: a case-control study.Scientific reports · 2026Article
- Cell-free DNA in sepsis: from molecular insights to clinical management.Military Medical Research · 2025Review
- The predictive ability of blood-based biomarkers to detect bacteremia in hospitalized neonatal foals.Veterinary journal (London, England : 1997) · 2025Article
- Extracted Plasma Cell-Free DNA Concentrations Are Elevated in Colic Patients with Systemic Inflammation.Veterinary sciences · 2024Article
- Distinct ecological fitness factors coordinated by a conservedProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Observational
- Plasma cfDNA predictors of established bacteraemic infection.Access microbiology · 2022Article
- Severity Biomarkers in Puumala Hantavirus Infection.Viruses · 2021Review
- Utility of cell-free DNA concentrations and illness severity scores to predict survival in critically ill neonatal foals.PloS one · 2021Article
- The Value of Serum Cell-Free DNA Levels in Patients With Schizophrenia.Frontiers in psychiatry · 2021Article
- Lung-protective ventilation suppresses systemic and hepatic vein levels of cell-free DNA in porcine experimental post-operative sepsis.BMC pulmonary medicine · 2020Article
- Plasma cell-free DNA predicts pediatric cerebral malaria severity.JCI insight · 2020Article
- Prognostic Power of Pathogen Cell-Free DNA inOpen forum infectious diseases · 2019Article
- Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With ComplicatedOpen forum infectious diseases · 2019Article
- Article
- Effect of sample type on plasma concentrations of cell-free DNA and nucleosomes in dogs.Veterinary record open · 2019Article
- Use of plasma mitochondrial DNA levels for determining disease severity and prognosis in pediatric sepsis: a case control study.BMC pediatrics · 2018Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionRecent studies have shown that apoptosis plays a critical role in the pathogenesis of sepsis. High plasma cell free DNA (cf-DNA) concentrations have been shown to be associated with sepsis outcome. The origin of cf-DNA is unclear.
methodsTotal plasma cf-DNA was quantified directly in plasma and the amplifiable cf-DNA assessed using quantitative PCR in 132 patients with bacteremia caused by Staphylococcus aureus, Streptococcus pneumoniae, ß-hemolytic streptococcae or Escherichia coli. The quality of cf-DNA was analyzed with a DNA Chip assay performed on 8 survivors and 8 nonsurvivors. Values were measured on days 1-4 after positive blood culture, on day 5-17 and on recovery.
resultsThe maximum cf-DNA values on days 1-4 (n = 132) were markedly higher in nonsurvivors compared to survivors (2.03 vs 1.26 ug/ml, p<0.001) and the AUCROC in the prediction of case fatality was 0.81 (95% CI 0.69-0.94). cf-DNA at a cut-off level of 1.52 ug/ml showed 83% sensitivity and 79% specificity for fatal disease. High cf-DNA (>1.52 ug/ml) remained an independent risk factor for case fatality in a logistic regression model. Qualitative analysis of cf-DNA showed that cf-DNA displayed a predominating low-molecular-weight cf-DNA band (150-200 bp) in nonsurvivors, corresponding to the size of the apoptotic nucleosomal DNA. cf-DNA concentration showed a significant positive correlation with visually graded apoptotic band intensity (R = 0.822, p<0.001).
conclusionsPlasma cf-DNA concentration proved to be a specific independent prognostic biomarker in bacteremia. cf-DNA displayed a predominating low-molecular-weight cf-DNA band in nonsurvivors corresponding to the size of apoptotic nucleosomal DNA.
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