ArticlePloS one2011
Lipid modifications of Sonic hedgehog ligand dictate cellular reception and signal response.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- The SLC-ome of membrane transport: from molecular discovery to physiology and clinical applications.Physiological reviews · 2026Review
- In vitro reconstitution of vertebrate Sonic Hedgehog protein cholesterolysis.bioRxiv : the preprint server for biology · 2026Article
- Gestational iron deficiency affects the ratio between interneuron subtypes in the postnatal cerebral cortex in mice.Development (Cambridge, England) · 2023Article
- A mathematical model of the role of aggregation in sonic hedgehog signalling.PLoS computational biology · 2021Article
- Specialized Intercellular Communications via Cytonemes and Nanotubes.Annual review of cell and developmental biology · 2018Review
- Loss-of-function mutations in FGF8 can be independent risk factors for holoprosencephaly.Human molecular genetics · 2018Article
- Cadherin-7 enhances Sonic Hedgehog signalling by preventing Gli3 repressor formation during neural tube patterning.Open biology · 2017Article
- Hedgehog Proteins Consume Steroidal CYP17A1 Antagonists: Potential Therapeutic Significance in Advanced Prostate Cancer.ChemMedChem · 2016Article
- Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway.Scientific reports · 2016Article
- Review
- Design and characterization of a photo-activatable hedgehog probe that mimics the natural lipidated form.Archives of biochemistry and biophysics · 2015Article
- Temozolomide resistance in glioblastoma occurs by miRNA-9-targeted PTCH1, independent of sonic hedgehog level.Oncotarget · 2015Article
- Arsenic inhibits hedgehog signaling during P19 cell differentiation.Toxicology and applied pharmacology · 2014Article
- A mutation in the mouse ttc26 gene leads to impaired hedgehog signaling.PLoS genetics · 2014Article
- Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling.Journal of neuro-oncology · 2014Article
- Specialized filopodia: at the 'tip' of morphogen transport and vertebrate tissue patterning.Current opinion in genetics & development · 2014Review
- Clinical, genetic and imaging findings identify new causes for corpus callosum development syndromes.Brain : a journal of neurology · 2014Review
- Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation.Cancer letters · 2013Article
- Lipids and prostate cancer.Prostaglandins & other lipid mediators · 2012Review
- Targeting protein lipidation in disease.Trends in molecular medicine · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSonic hedgehog (Shh) signaling regulates cell growth during embryonic development, tissue homeostasis and tumorigenesis. Concentration-dependent cellular responses to secreted Shh protein are essential for tissue patterning. Shh ligand is covalently modified by two lipid moieties, cholesterol and palmitate, and their hydrophobic properties are known to govern the cellular release and formation of soluble multimeric Shh complexes. However, the influences of the lipid moieties on cellular reception and signal response are not well understood. METHODOLOGY/PRINCIPAL
findingsWe analyzed fully lipidated Shh and mutant forms to eliminate one or both adducts in NIH3T3 mouse embryonic fibroblasts. Quantitative measurements of recombinant Shh protein concentration, cellular localization, and signaling potency were integrated to determine the contributions of each lipid adduct on ligand cellular localization and signaling potency. We demonstrate that lipid modification is required for cell reception, that either adduct is sufficient to confer cellular association, that the cholesterol adduct anchors ligand to the plasma membrane and that the palmitate adduct augments ligand internalization. We further show that signaling potency correlates directly with cellular concentration of Shh ligand. CONCLUSIONS/SIGNIFICANCE: The findings of this study demonstrate that lipid modification of Shh determines cell concentration and potency, revealing complementary functions of hydrophobic modification in morphogen signaling by attenuating cellular release and augmenting reception of Shh protein in target tissues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.