ArticleFrontiers in microbiology2011
Conidial Dihydroxynaphthalene Melanin of the Human Pathogenic Fungus Aspergillus fumigatus Interferes with the Host Endocytosis Pathway.
Article in Frontiers in microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
85 citing papers in PubMed, 164 citations in OpenAlex.
- Review
- Macrophage vacuolar ATPase (v-ATPase) function controlsbioRxiv : the preprint server for biology · 2025Article
- Article
- Antifungal Innate Immunity.Advances in experimental medicine and biology · 2025Review
- Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation.Nature microbiology · 2024Article
- Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing.Nature communications · 2024Article
- Polyketide Trimming Shapes Dihydroxynaphthalene-Melanin and Anthraquinone Pigments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Melanin depletion affects Aspergillus flavus conidial surface proteins, architecture, and virulence.Applied microbiology and biotechnology · 2024Article
- Accelerating the understanding ofCurrent research in microbial sciences · 2024Review
- Two zinc finger proteins, VdZFP1 and VdZFP2, interact with VdCmr1 to promote melanized microsclerotia development and stress tolerance in Verticillium dahliae.BMC biology · 2023Article
- Fungi's Swiss Army Knife: Pleiotropic Effect of Melanin in Fungal Pathogenesis during Cattle Mycosis.Journal of fungi (Basel, Switzerland) · 2023Article
- Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages.PLoS neglected tropical diseases · 2023Article
- Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway.Cell host & microbe · 2023Article
- "Under Pressure" - How fungi evade, exploit, and modulate cells of the innate immune system.Seminars in immunology · 2023Review
- Article
- Targeting of phagolysosomes containing conidia of the fungus Aspergillus fumigatus with polymeric particles.Applied microbiology and biotechnology · 2023Article
- Conidium Specific Polysaccharides inJournal of fungi (Basel, Switzerland) · 2023Article
- Deciphering the Role of PIG1 and DHN-Melanin inJournal of fungi (Basel, Switzerland) · 2023Article
- Fungal extracellular vesicle-mediated regulation: from virulence factor to clinical application.Frontiers in microbiology · 2023Review
- The nature of the fungal cargo induces significantly different temporal programmes of macrophage phagocytosis.Cell surface (Amsterdam, Netherlands) · 2022Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus fumigatus is the most important air-borne fungal pathogen of humans. The interaction of the pathogen with the host's immune system represents a key process to understand pathogenicity. For elimination of invading microorganisms, they need to be efficiently phagocytosed and located in acidified phagolysosomes. However, as shown previously, A. fumigatus is able to manipulate the formation of functional phagolysosomes. Here, we demonstrate that in contrast to pigmentless pksP mutant conidia of A. fumigatus, the gray-green wild-type conidia inhibit the acidification of phagolysosomes of alveolar macrophages, monocyte-derived macrophages, and human neutrophil granulocytes. Therefore, this inhibition is independent of the cell type and applies to the major immune effector cells required for defense against A. fumigatus. Studies with melanin ghosts indicate that the inhibitory effect of wild-type conidia is due to their dihydroxynaphthalene (DHN)-melanin covering the conidia, whereas the hydrophobin RodA rodlet layer plays no role in this process. This is also supported by the observation that pksP conidia still exhibit the RodA hydrophobin layer, as shown by scanning electron microscopy. Mutants defective in different steps of the DHN-melanin biosynthesis showed stronger inhibition than pksP mutant conidia but lower inhibition than wild-type conidia. Moreover, A. fumigatus and A. flavus led to a stronger inhibition of phagolysosomal acidification than A. nidulans and A. terreus. These data indicate that a certain type of DHN-melanin that is different in the various Aspergillus species, is required for maximal inhibition of phagolysosomal acidification. Finally, we identified the vacuolar ATPase (vATPase) as potential target for A. fumigatus based on the finding that addition of bafilomycin which inhibits vATPase, led to complete inhibition of the acidification whereas the fusion of phagosomes containing wild-type conidia and lysosomes was not affected.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.