ArticleFrontiers in microbiology2011
A Novel in vitro Human Macrophage Model to Study the Persistence of Mycobacterium tuberculosis Using Vitamin D(3) and Retinoic Acid Activated THP-1 Macrophages.
Article in Frontiers in microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
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- Delineating transcriptional crosstalk between Mycobacterium avium subsp. paratuberculosis and human THP-1 cells at the early stage of infection via dual RNA-seq analysis.Veterinary research · 2022Article
- Recombinant Human Lactoferrin Reduces Inflammation and Increases Fluoroquinolone Penetration to Primary Granulomas During Mycobacterial Infection of C57Bl/6 Mice.Archivum immunologiae et therapiae experimentalis · 2022Article
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- Mesenchymal Stem Cells and Tuberculosis: Clinical Challenges and Opportunities.Frontiers in immunology · 2021Review
- A Low-Prevalence Single-Nucleotide Polymorphism in the Sensor Kinase PhoR inFrontiers in microbiology · 2021Article
- Immunomodulatory Effects of RecombinantPathogens (Basel, Switzerland) · 2020Article
- Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus.Scientific reports · 2020Article
- The Pathogenesis of Tuberculosis-The Koch Phenomenon Reinstated.Pathogens (Basel, Switzerland) · 2020Review
- Association of Dietary Micronutrient Intake with Pulmonary Tuberculosis Treatment Failure Rate: ACohort Study.Nutrients · 2020Article
- Vitamin D-Cathelicidin Axis: at the Crossroads between Protective Immunity and Pathological Inflammation during Infection.Immune network · 2020Review
- Targeting Autophagy in Innate Immune Cells: Angel or Demon During Infection and Vaccination?Frontiers in immunology · 2020Review
- Foam Cells ControlFrontiers in microbiology · 2020Article
- Vitamin D modulates human macrophage response to Mycobacterium tuberculosis DNA.Tuberculosis (Edinburgh, Scotland) · 2019Article
- Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy.Scientific reports · 2017Article
- Experimental study of tuberculosis: From animal models to complex cell systems and organoids.PLoS pathogens · 2017Review
- A responsive human triple-culture model of the air-blood barrier: incorporation of different macrophage phenotypes.Journal of tissue engineering and regenerative medicine · 2017Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Mycobacterium tuberculosis (Mtb) replicates within the human macrophages and we investigated the activating effects of retinoic acid (RA) and vitamin D(3) (VD) on macrophages in relation to the viability of intracellular Mtb. A combination of these vitamins (RAVD) enhanced the levels of DC-SIGN and mannose receptors on THP-1 macrophages that increased mycobacterial uptake but inhibited the subsequent intracellular growth of Mtb by inducing reactive oxygen species and autophagy. RAVD also enhanced antigen presenting and chemotactic receptors on THPs suggesting an activated phenotype for RAVD activated THPs. RAVD mediated activation was also associated with a marked phenotypic change in Mtb infected THPs that fused with adjacent THPs to form multinucleated giant cells (MNGCs). Typically, MNGCs occurred over 30 days of in vitro culture and contained non-replicating persisting Mtb for more than 60 days in culture. Latent tuberculosis occurs in over a third of mankind and we propose that RAVD mediated induction of persistent Mtb within human macrophages provides a novel model to develop therapeutic approaches and investigate pathogenesis of latency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.