ArticleInternational journal of molecular sciences2011
Proliferation-attenuating and apoptosis-inducing effects of tryptanthrin on human chronic myeloid leukemia K562 cell line in vitro.
Article in International journal of molecular sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Chemistry and Biological Activity of 11Molecules (Basel, Switzerland) · 2026Review
- De novo synthesis of tryptanthrin and its derivatives from indole: engineering a sustainable photocatalytic strategy.Molecular diversity · 2026Article
- Translating molecular insights into clinical success: alkaloid-based therapies for leukemia.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Therapeutic Potential of Natural Compounds to Modulate WNT/β-Catenin Signaling in Cancer: Current State of Art and Challenges.International journal of molecular sciences · 2024Review
- Recent advances of tryptanthrin and its derivatives as potential anticancer agents.RSC medicinal chemistry · 2024Review
- Tryptanthrin inhibits tumor angiogenesis via Notch/Dll4 signaling pathway in zebrafish.Translational cancer research · 2023Article
- Discovery of Tryptanthrin and Its Derivatives and Its Activities against NSCLC In Vitro via Both Apoptosis and Autophagy Pathways.International journal of molecular sciences · 2023Article
- Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth ofMolecules (Basel, Switzerland) · 2022Article
- Pharmacological attenuation of melanoma by tryptanthrin pertains to the suppression of MITF-M through MEK/ERK signaling axis.Cellular and molecular life sciences : CMLS · 2022Article
- The Natural Alkaloid Tryptanthrin Induces Apoptosis-like Death inTropical medicine and infectious disease · 2022Article
- Identification of Spiro-Fused Pyrrolo[3,4-International journal of molecular sciences · 2021Article
- Pulsed Thermal Method for Monitoring Cell Proliferation in Real-Time.Sensors (Basel, Switzerland) · 2021Article
- Cancer Chemoprevention: A Strategic Approach Using Phytochemicals.Frontiers in pharmacology · 2021Review
- Review
- Antiviral Action of Tryptanthrin Isolated fromBiomolecules · 2020Article
- Biologically active quinoline and quinazoline alkaloids part II.Medicinal research reviews · 2018Review
- Suppression of VEGF-induced angiogenesis and tumor growth by Eugenia jambolana, Musa paradisiaca, and Coccinia indica extracts.Pharmaceutical biology · 2017Article
- Study on pharmacokinetics and tissue distribution of single dose oral tryptanthrin in Kunming mice by validated reversed-phase high-performance liquid chromatography with ultraviolet detection.Integrative medicine research · 2017Article
- Characterization of anti-leukemia components from Indigo naturalis using comprehensive two-dimensional K562/cell membrane chromatography and in silico target identification.Scientific reports · 2016Article
- DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria.Scientific reports · 2015Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Tryptanthrin, a kind of indole quinazoline alkaloid, has been shown to exhibit anti-microbial, anti-inflammation and anti-tumor effects both in vivo and in vitro. However, its biological activity on human chronic myeloid leukemia cell line K562 is not fully understood. In the present study, we investigated the proliferation-attenuating and apoptosis-inducing effects of tryptanthrin on leukemia K562 cells in vitro and explored the underlying mechanisms. The results showed that tryptanthrin could significantly inhibit K562 cells proliferation in a time- and dose-dependent manner as evidenced by MTT assay and flow cytometry analysis. We also observed pyknosis, chromatin margination and the formation of apoptotic bodies in the presence of tryptanthrin under the electron microscope. Nuclei fragmentation and condensation by Hoechst 33258 staining were detected as well. The amount of apoptotic cells significantly increased whereas the mitochondrial membrane potential decreased dramatically after tryptanthrin exposure. K562 cells in the tryptanthrin treated group exhibited an increase in cytosol cyt-c, Bax and activated caspase-3 expression while a decrease in Bcl-2, mito cyt-c and pro-caspase-3 contents. However, the changes of pro-caspase-3 and activated caspase-3 could be abolished by a pan-caspase inhibitor ZVAD-FMK. These results suggest that tryptanthrin has proliferation-attenuating and apoptosis-inducing effects on K562 cells. The underlying mechanism is probably attributed to the reduction in mitochondria membrane potential, the release of mito cyt-c and pro-caspase-3 activation.
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