ArticleCurrent genetics1990
G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae.
Article in Current genetics, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 75 citations in OpenAlex.
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae.PLoS genetics · 2023Article
- Multiple Antibiotic Resistance Plasmids Allow Scalable, PCR-Mediated DNA Manipulation and Near-Zero Background Cloning.Food technology and biotechnology · 2016Article
- Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae.Microbial cell factories · 2013Article
- New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae.G3 (Bethesda, Md.) · 2012Article
- Oxygen-regulated isoforms of cytochrome c oxidase have differential effects on its nitric oxide production and on hypoxic signaling.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity.Applied and environmental microbiology · 2005Article
- The spoilage yeast Zygosaccharomyces bailii forms mitotic spores: a screening method for haploidization.Applied and environmental microbiology · 2003Article
- The target of rapamycin signaling pathway regulates mRNA turnover in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2001Article
- Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough.Applied and environmental microbiology · 1999Article
- Silencing MIG1 in Saccharomyces cerevisiae: effects of antisense MIG1 expression and MIG1 gene disruption.Applied and environmental microbiology · 1997Article
- Development of a high-performance liquid chromatographic assay for G418 sulfate (Geneticin).Antimicrobial agents and chemotherapy · 1997Article
- Alleviation of glucose repression of maltose metabolism by MIG1 disruption in Saccharomyces cerevisiae.Applied and environmental microbiology · 1996Article
- Determination of chromosome copy numbers in Saccharomyces cerevisiae strains via integrative probe and blot hybridization techniques.Current genetics · 1995Article
- Cloning of Candida boidinii DNA fragments promoting autonomous replication of plasmids in Saccharomyces cerevisiae.Folia microbiologica · 1992Article
- DMSO-enhanced whole cell yeast transformation.Nucleic acids research · 1991Article
- Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Cycloheximide resistance as a yeast cloning marker.Current genetics · 1991Article
- The absence of a mitochondrial genome in rho0 yeast cells extends lifespan independently of retrograde regulation.Experimental gerontologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coding sequence cartridges for aminoglycoside phosphotransferase (APT) were isolated from bacterial transposon Tn903. When incorporated into a heterologous gene construction utilising the PGK1 promoter and terminator, the heterologous APT gene provided a G418-resistance determinant that functioned efficiently as a dominant marker for yeast in both multiple- and single-copy. Transformant colonies on selective medium appeared rapidly, within 36-48 h, and growth rate of the transformed cells was normal. A simple and highly sensitive radiolabelling assay for APT enzyme activity was developed for use with crude cell protein extracts. Enzyme activity units were equated to the amount of APT protein present in the cells, and the APT protein was shown to be stable in yeast. Heterologous APT expression was 130-fold reduced compared with homologous PGK1. This resulted from an estimated two-fold decrease in mRNA level and a 65-fold decrease in translation efficiency. The latter was unaffected by AUG sequence context change, but corresponded with a high frequency of minor codons in the APT-coding sequence. APT can be used as a semi-quantitative reporter of gene expression, whose useful features are in vivo detection via the G418-resistance phenotype and powerful cell-free assay.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.