ArticleJournal of immunology (Baltimore, Md. : 1950)2011
Selection of individual VH genes occurs at the pro-B to pre-B cell transition.
Article in Journal of immunology (Baltimore, Md. : 1950), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Adaptive Immune Receptor Repertoire (AIRR) Community Guide to TR and IG Gene Annotation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Bayesian optimist's guide to adaptive immune receptor repertoire analysis.Immunological reviews · 2018Review
- On being the right size: antibody repertoire formation in the mouse and human.Immunogenetics · 2018Review
- The analysis of clonal expansions in normal and autoimmune B cell repertoires.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Antibody repertoire deep sequencing reveals antigen-independent selection in maturing B cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RAG2 mutants alter DSB repair pathway choice in vivo and illuminate the nature of 'alternative NHEJ'.Nucleic acids research · 2014Article
- The restricted DH gene reading frame usage in the expressed human antibody repertoire is selected based upon its amino acid content.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity.The Journal of experimental medicine · 2013Article
- B-cell tolerance defects in the B6.Aec1/2 mouse model of Sjögren's syndrome.Journal of clinical immunology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
B cells are subjected to selection at multiple checkpoints during their development. The selection of Ab H chains is difficult to study because of the large diversity of the CDR3. To study the selection of individual Ab H chain V region genes (V(H)), we performed CDR3 spectratyping of ∼ 75-300 rearrangements per individual V(H) in C57BL6/J mice. We measured the fraction of rearrangements that were in-frame in B cell DNA. We demonstrate that individual V(H)s have different fractions of in-frame rearrangements (IF fractions) ranging from 10 to 90% and that these IF fractions are reproducible in different mice. For most V(H)s, the IF fraction in pro-B cells approximated 33% and then shifted to the nearly final (mature) B cell value by the cycling pre-B cell stage. The frequency of high in-frame (IF) V(H) usage increased in cycling pre-B cells compared with that in pro-B cells, whereas this did not occur for low IF V(H)s. The IF fraction did not shift as much in BCR-expressing B cells and was minimally affected by L chain usage for most V(H). High IF clan II/III V(H)s share more positively charged CDR2 sequences, whereas high IF clan I J558 CDR2 sequences are diverse. These data indicate that individual V(H)s are subjected to differential selection, that V(H) IF fraction is mainly established through pre-BCR-mediated selection, that it may operate differently in clan I versus II/III V(H)s, and that it has a lasting influence on the Ab repertoire.
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Registered trials
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