ArticleAntimicrobial agents and chemotherapy2011
Superoxide dismutases are involved in Candida albicans biofilm persistence against miconazole.
Article in Antimicrobial agents and chemotherapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans.BMC microbiology · 2024Pooled it
- Repositioning Antimicrobial Peptides Against WHO-Priority Fungi.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Persister cells in human fungal pathogens.PLoS pathogens · 2025Review
- Antifungal persistence: Clinical relevance and mechanisms.PLoS pathogens · 2025Review
- Fungal biofilms in human health and disease.Nature reviews. Microbiology · 2025Review
- Transcriptome Analysis ofMicroorganisms · 2024Article
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- Exploring therapeutic avenues: mesenchymal stem/stromal cells and exosomes in confronting enigmatic biofilm-producing fungi.Archives of microbiology · 2023Review
- Anti-Biofilm Activity of Cocultimycin A againstInternational journal of molecular sciences · 2023Article
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- Synergistic activity of pomegranate rind extract and Zn (II) against Candida albicans under planktonic and biofilm conditions, and a mechanistic insight based upon intracellular ROS induction.Scientific reports · 2022Article
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- Antifungal Effect of Piezoelectric Charges on PMMA Dentures.ACS biomaterials science & engineering · 2021Article
- The Glycosylphosphatidylinositol-Anchored Superoxide Dismutase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Approaches for Mitigating Microbial Biofilm-Related Drug Resistance: A Focus on Micro- and Nanotechnologies.Molecules (Basel, Switzerland) · 2021Review
- Transcription Factors of CAT1, EFG1, and BCR1 Are Effective in Persister Cells ofFrontiers in microbiology · 2021Article
- Marine-Derived Compounds and Prospects for Their Antifungal Application.Molecules (Basel, Switzerland) · 2020Review
- Bacterial persisters in long-term infection: Emergence and fitness in a complex host environment.PLoS pathogens · 2020Article
- Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the cellular mechanisms responsible for the occurrence of miconazole-tolerant persisters in Candida albicans biofilms. Miconazole induced about 30% killing of sessile C. albicans cells at 75 μM. The fraction of miconazole-tolerant persisters, i.e., cells that can survive high doses of miconazole (0.6 to 2.4 mM), in these biofilms was 1 to 2%. Since miconazole induces reactive oxygen species (ROS) in sessile C. albicans cells, we focused on a role for superoxide dismutases (Sods) in persistence and found the expression of Sod-encoding genes in sessile C. albicans cells induced by miconazole compared to the expression levels in untreated sessile C. albicans cells. Moreover, addition of the superoxide dismutase inhibitor N,N'-diethyldithiocarbamate (DDC) to C. albicans biofilms resulted in an 18-fold reduction of the miconazole-tolerant persister fraction and in increased endogenous ROS levels in these cells. Treatment of biofilms of C. albicans clinical isolates with DDC resulted in an 18-fold to more than 200-fold reduction of their miconazole-tolerant persister fraction. To further confirm the important role for Sods in C. albicans biofilm persistence, we used a Δsod4 Δsod5 mutant lacking Sods 4 and 5. Biofilms of the Δsod4 Δsod5 mutant contained at least 3-fold less of the miconazole-tolerant persisters and had increased ROS levels compared to biofilms of the isogenic wild type (WT). In conclusion, the occurrence of miconazole-tolerant persisters in C. albicans biofilms is linked to the ROS-detoxifying activity of Sods. Moreover, Sod inhibitors can be used to potentiate the activity of miconazole against C. albicans biofilms.
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