ArticleProceedings of the National Academy of Sciences of the United States of America2011
Humanized mice with ectopic artificial liver tissues.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 157 citations in OpenAlex.
- Acellular lung matrix scaffold coated by fibronectin enhances hepatic differentiation of adipose-derived mesenchymal stem cells.Anatomy & cell biology · 2026Article
- Synthetic control of implanted engineered liver tissue growth.Science advances · 2026Article
- Bioprinted platform for parallelized screening of engineered microtissues in vivo.Cell stem cell · 2025Article
- Investigation of Biotransformation Pathways in a Chimeric Mouse with a Humanized Liver.International journal of molecular sciences · 2025Article
- 3D Bioprinting for Engineered Tissue Constructs and Patient-Specific Models: Current Progress and Prospects in Clinical Applications.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Three Dimensional Bioprinting for Hepatic Tissue Engineering: From In Vitro Models to Clinical Applications.Tissue engineering and regenerative medicine · 2024Review
- Nanofabrication Technologies to Control Cell and Tissue Function in Three-Dimension.Gels (Basel, Switzerland) · 2023Review
- Extracellular Microenvironmental Control for Organoid Assembly.Tissue engineering. Part B, Reviews · 2022Review
- Cell and Tissue Therapy for the Treatment of Chronic Liver Disease.Annual review of biomedical engineering · 2021Review
- Current status of hepatocyte-like cell therapy from stem cells.Surgery today · 2021Review
- Gaining New Biological and Therapeutic Applications into the Liver with 3D In Vitro Liver Models.Tissue engineering and regenerative medicine · 2020Review
- Controlled Apoptosis of Stromal Cells to Engineer Human Microlivers.Advanced functional materials · 2020Article
- Solid Organ Bioprinting: Strategies to Achieve Organ Function.Chemical reviews · 2020Review
- Assessing the compatibility of primary human hepatocyte culture within porous silk sponges.RSC advances · 2020Article
- A Hepatic Scaffold from Decellularized Liver Tissue: Food for Thought.Biomolecules · 2019Review
- Long-Term Engineered Cultures of Primary Mouse Hepatocytes for Strain and Species Comparison Studies During Drug Development.Gene expression · 2019Article
- A novel evaluation system for whole-organ-engineered liver graft by ex vivo application to a highly reproducible hepatic failure rat model.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2019Article
- Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages.Cell transplantation · 2019Article
- Construction of Pedicled Smooth Muscle Tissues by Combining the Capsule Tissue and Cell Sheet Engineering.Cell transplantation · 2019Article
- Bioengineered functional humanized livers: An emerging supportive modality to bridge the gap of organ transplantation for management of end-stage liver diseases.World journal of hepatology · 2018Review
14 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
"Humanized" mice offer a window into aspects of human physiology that are otherwise inaccessible. The best available methods for liver humanization rely on cell transplantation into immunodeficient mice with liver injury but these methods have not gained widespread use due to the duration and variability of hepatocyte repopulation. In light of the significant progress that has been achieved in clinical cell transplantation through tissue engineering, we sought to develop a humanized mouse model based on the facile and ectopic implantation of a tissue-engineered human liver. These human ectopic artificial livers (HEALs) stabilize the function of cryopreserved primary human hepatocytes through juxtacrine and paracrine signals in polymeric scaffolds. In contrast to current methods, HEALs can be efficiently established in immunocompetent mice with normal liver function. Mice transplanted with HEALs exhibit humanized liver functions persistent for weeks, including synthesis of human proteins, human drug metabolism, drug-drug interaction, and drug-induced liver injury. Here, mice with HEALs are used to predict the disproportionate metabolism and toxicity of "major" human metabolites using multiple routes of administration and monitoring. These advances may enable manufacturing of reproducible in vivo models for diverse drug development and research applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.