ArticleNucleic acids research2011
Reconstitution of a functional IS608 single-strand transpososome: role of non-canonical base pairing.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors.Nature biotechnology · 2024Article
- Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope.Nature communications · 2024Article
- Phylogenetic Relationships among TnpB-Containing Mobile Elements in Six Bacterial Species.Genes · 2023Article
- Single-strand DNA processing: phylogenomics and sequence diversity of a superfamily of potential prokaryotic HuH endonucleases.BMC genomics · 2018Article
- Targeting IS608 transposon integration to highly specific sequences by structure-based transposon engineering.Nucleic acids research · 2018Article
- Single strand transposition at the host replication fork.Nucleic acids research · 2016Article
- Real-time transposable element activity in individual live cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- 3dRNAscore: a distance and torsion angle dependent evaluation function of 3D RNA structures.Nucleic acids research · 2015Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Survey of chimeric IStron elements in bacterial genomes: multiple molecular symbioses between group I intron ribozymes and DNA transposons.Nucleic acids research · 2014Article
- Bacterial insertion sequences: their genomic impact and diversity.FEMS microbiology reviews · 2014Review
- Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.Nature reviews. Microbiology · 2013Review
- IS200/IS605 family single-strand transposition: mechanism of IS608 strand transfer.Nucleic acids research · 2013Article
- The emerging diversity of transpososome architectures.Quarterly reviews of biophysics · 2012Review
- Structuring the bacterial genome: Y1-transposases associated with REP-BIME sequences.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Single-stranded (ss) transposition, a recently identified mechanism adopted by members of the widespread IS200/IS605 family of insertion sequences (IS), is catalysed by the transposase, TnpA. The transposase of IS608, recognizes subterminal imperfect palindromes (IP) at both IS ends and cleaves at sites located at some distance. The cleavage sites, C, are not recognized directly by the protein but by short sequences 5' to the foot of each IP, guide (G) sequences, using a network of canonical ('Watson-Crick') base interactions. In addition a set of non-canonical base interactions similar to those found in RNA structures are also involved. We have reconstituted a biologically relevant complex, the transpososome, including both left and right ends and TnpA, which catalyses excision of a ss DNA circle intermediate. We provide a detailed picture of the way in which the IS608 transpososome is assembled and demonstrate that both C and G sequences are essential for forming a robust transpososome detectable by EMSA. We also address several questions central to the organization and function of the ss transpososome and demonstrate the essential role of non-canonical base interactions in the IS608 ends for its stability by using point mutations which destroy individual non-canonical base interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.