ArticleBMC biotechnology2011
Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation.
Article in BMC biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 75 citations in OpenAlex.
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- Establishing a Cre/loxP-based genetic manipulation system for Acanthamoeba: Targeted genome editing and stable reporter expression.Parasites, hosts and diseases · 2025Article
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- Transcription factor bHLH121 regulates root cortical aerenchyma formation in maize.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation.Transgenic research · 2022Article
- Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA-sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco.Plant cell reports · 2022Article
- Efficient Gene Stacking in Rice Using the GAANTRY System.Rice (New York, N.Y.) · 2021Article
- Transcription Terminator-Mediated Enhancement in Transgene Expression in Maize: Preponderance of the AUGAAU Motif Overlapping With Poly(A) Signals.Frontiers in plant science · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Identification of functional single nucleotide polymorphism ofPlant direct · 2020Article
- Engineering linear, branched-chain triterpene metabolism in monocots.Plant biotechnology journal · 2019Article
- A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants.Frontiers in plant science · 2019Review
- A profilin gene promoter from switchgrass (Panicum virgatum L.) directs strong and specific transgene expression to vascular bundles in rice.Plant cell reports · 2018Article
- Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis.Nature biotechnology · 2018Article
- Switchgrass (Biotechnology for biofuels · 2018Article
- A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.The Plant cell · 2017Article
- Expression in grasses of multiple transgenes for degradation of munitions compounds on live-fire training ranges.Plant biotechnology journal · 2017Article
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L.) ubiquitin genes (PvUbi1 and PvUbi2) were cloned and characterized. Reporter constructs were produced containing the isolated 5' upstream regulatory regions of the coding sequences (i.e. PvUbi1 and PvUbi2 promoters) fused to the uidA coding region (GUS) and tested for transient and stable expression in a variety of plant species and tissues.
resultsPvUbi1 consists of 607 bp containing cis-acting regulatory elements, a 5' untranslated region (UTR) containing a 93 bp non-coding exon and a 1291 bp intron, and a 918 bp open reading frame (ORF) that encodes four tandem, head -to-tail ubiquitin monomer repeats followed by a 191 bp 3' UTR. PvUbi2 consists of 692 bp containing cis-acting regulatory elements, a 5' UTR containing a 97 bp non-coding exon and a 1072 bp intron, a 1146 bp ORF that encodes five tandem ubiquitin monomer repeats and a 183 bp 3' UTR. PvUbi1 and PvUbi2 were expressed in all examined switchgrass tissues as measured by qRT-PCR. Using biolistic bombardment, PvUbi1 and PvUbi2 promoters showed strong expression in switchgrass and rice callus, equaling or surpassing the expression levels of the CaMV 35S, 2x35S, ZmUbi1, and OsAct1 promoters. GUS staining following stable transformation in rice demonstrated that the PvUbi1 and PvUbi2 promoters drove expression in all examined tissues. When stably transformed into tobacco (Nicotiana tabacum), the PvUbi2+3 and PvUbi2+9 promoter fusion variants showed expression in vascular and reproductive tissues.
conclusionsThe PvUbi1 and PvUbi2 promoters drive expression in switchgrass, rice and tobacco and are strong constitutive promoter candidates that will be useful in genetic transformation of monocots and dicots.
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