ArticleJournal of translational medicine2011
A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols.
Article in Journal of translational medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.Frontiers in immunology · 2021Article
- Optimization in Detection of Antigen-Specific T Cells Through Differentially Labeled MHC Multimers.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Adhering to adhesion: assessing integrin conformation to monitor T cells.Cancer immunology, immunotherapy : CII · 2019Review
- Modulation of CRTh2 expression on allergen-specific T cells following peptide immunotherapy.Allergy · 2019Article
- Current status and perspectives in translational biomarker research for PD-1/PD-L1 immune checkpoint blockade therapy.Journal of hematology & oncology · 2016Review
- Validation of biomarkers to predict response to immunotherapy in cancer: Volume I - pre-analytical and analytical validation.Journal for immunotherapy of cancer · 2016Review
- Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters.Cancer immunology, immunotherapy : CII · 2015Article
- Cryopreservation of MHC multimers: Recommendations for quality assurance in detection of antigen specific T cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Managing Multi-center Flow Cytometry Data for Immune Monitoring.Cancer informatics · 2014Article
- CIMT 2014: Next waves in cancer immunotherapy--report on the 12th annual meeting of the Association for Cancer Immunotherapy: May 6–8 2014, Mainz, Germany.Human vaccines & immunotherapeutics · 2014Article
- Immunological Monitoring to Rationally Guide AAV Gene Therapy.Frontiers in immunology · 2013Review
- Structured reporting of T cell assay results.Cancer immunity · 2013Article
- The immuno-oncology framework: Enabling a new era of cancer therapy.Oncoimmunology · 2012Article
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Authors and funding
13 authors.
Funding
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Abstract
backgroundThe introduction of antibody markers to identify undesired cell populations in flow-cytometry based assays, so called DUMP channel markers, has become a practice in an increasing number of labs performing HLA-peptide multimer assays. However, the impact of the introduction of a DUMP channel in multimer assays has so far not been systematically investigated across a broad variety of protocols.
methodsThe Cancer Research Institute's Cancer Immunotherapy Consortium (CRI-CIC) conducted a multimer proficiency panel with a specific focus on the impact of DUMP channel use. The panel design allowed individual laboratories to use their own protocol for thawing, staining, gating, and data analysis. Each experiment was performed twice and in parallel, with and without the application of a dump channel strategy.
resultsThe introduction of a DUMP channel is an effective measure to reduce the amount of non-specific MULTIMER binding to T cells. Beneficial effects for the use of a DUMP channel were observed across a wide range of individual laboratories and for all tested donor-antigen combinations. In 48% of experiments we observed a reduction of the background MULTIMER-binding. In this subgroup of experiments the median background reduction observed after introduction of a DUMP channel was 0.053%.
conclusionsWe conclude that appropriate use of a DUMP channel can significantly reduce background staining across a large fraction of protocols and improve the ability to accurately detect and quantify the frequency of antigen-specific T cells by multimer reagents. Thus, use of a DUMP channel may become crucial for detecting low frequency antigen-specific immune responses. Further recommendations on assay performance and data presentation guidelines for publication of MULTIMER experimental data are provided.
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