ArticleJournal of the American Chemical Society2011
The products of 5-fluorouridine by the action of the pseudouridine synthase TruB disfavor one mechanism and suggest another.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanistic insight into the pseudouridylation of RNA.RNA biology · 2025Review
- Improved Sampling of Adaptive Path Collective Variables by Stabilized Extended-System Dynamics.Journal of chemical theory and computation · 2023Article
- The Pseudouridine Synthases Proceed through a Glycal Intermediate.Journal of the American Chemical Society · 2016Article
- Accurate placement of substrate RNA by Gar1 in H/ACA RNA-guided pseudouridylation.Nucleic acids research · 2015Article
- Dye label interference with RNA modification reveals 5-fluorouridine as non-covalent inhibitor.Nucleic acids research · 2014Article
- An arginine-aspartate network in the active site of bacterial TruB is critical for catalyzing pseudouridine formation.Nucleic acids research · 2014Article
- RNA-guided isomerization of uridine to pseudouridine--pseudouridylation.RNA biology · 2014Review
- Pseudouridine: still mysterious, but never a fake (uridine)!RNA biology · 2014Review
- Pseudouridine monophosphate glycosidase: a new glycosidase mechanism.Biochemistry · 2012Article
- Kinetic and thermodynamic characterization of the reaction pathway of box H/ACA RNA-guided pseudouridine formation.Nucleic acids research · 2012Article
- Archaeal proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA.Scientific reports · 2012Article
- Article
- Unexpected linear ion trap collision-induced dissociation and Fourier transform ion cyclotron resonance infrared multi-photon dissociation fragmentation of a hydrated C-glycoside of 5-fluorouridine formed by the action of the pseudouridine synthases RluA and TruB.Rapid communications in mass spectrometry : RCM · 2011Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The pseudouridine synthase TruB handles 5-fluorouridine in RNA as a substrate, converting it into two isomeric hydrated products. Unexpectedly, the two products differ not in the hydrated pyrimidine ring but in the pentose ring, which is epimerized to arabinose in the minor product. This inversion of stereochemistry at C2' suggests that pseudouridine generation may proceed by a mechanism involving a glycal intermediate or that the previously proposed mechanism involving an acylal intermediate operates but with an added reaction manifold for 5-fluorouridine versus uridine. The arabino product strongly disfavors a mechanism involving a Michael addition to the pyrimidine ring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.