TRANSCRIPTIONAL AND PHOSPHO-PROTEOMIC SCREENS REVEAL STEM CELL ACTIVATION OF INSULIN-RESISTANCE AND TRANSFORMATION PATHWAYS FOLLOWING A SINGLE MINIMALLY TOXIC EPISODE OF ROS.

R Mouzannar, J McCafferty, G Benedetto, C Richardson

Open access · diamondAbstract read

In one paragraph

Article in International journal of genomics and proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it

0cells of the map it votes in

9citing papers in PubMed

0.6field-weighted citation impact, top 29% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

Effect of increasing glutathione with cysteine and glycine supplementation on mitochondrial fuel oxidation, insulin sensitivity, and body composition in older HIV-infected patients.The Journal of clinical endocrinology and metabolism · 2014
Trial
Supplementing Glycine and N-acetylcysteine (GlyNAC) in Aging HIV Patients Improves Oxidative Stress, Mitochondrial Dysfunction, Inflammation, Endothelial Dysfunction, Insulin Resistance, Genotoxicity, Strength, and Cognition: Results of an Open-Label Clinical Trial.Biomedicines · 2020
Article
Energy Stores, Oxidative Balance, and Sleep in Migratory Garden Warblers (Integrative organismal biology (Oxford, England) · 2020
Article
Real-Time HJournal of cellular biochemistry · 2017
Article
Ultraviolet Radiation-Induced Skin Aging: The Role of DNA Damage and Oxidative Stress in Epidermal Stem Cell Damage Mediated Skin Aging.Stem cells international · 2016
Review
Oxidative stress, bone marrow failure, and genome instability in hematopoietic stem cells.International journal of molecular sciences · 2015
Review
Roles of reactive oxygen species in the fate of stem cells.Antioxidants & redox signaling · 2014
Review
Long-term effects of chromatin remodeling and DNA damage in stem cells induced by environmental and dietary agents.Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer · 2013
Review
The regulation of food intake in mammalian hibernators: a review.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2012
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

R MouzannarUNC-Charlotte, Department of Biology and Bioinformatics Research Center, Charlotte, NC 28223.

J McCafferty

G Benedetto

C Richardson

University of North Carolina at Charlotte · US

Funding

Etiology of Translocations in Hematopoietic CellsR01CA100159 · NCI · UNIVERSITY OF NORTH CAROLINA CHARLOTTE · PI RICHARDSON, CHRISTINE A. · 2003 to 2017

$2.4M

Etiology of translocations in hematopoietic cellsR56CA100159 · NCI · UNIVERSITY OF NORTH CAROLINA CHARLOTTE · PI RICHARDSON, CHRISTINE A. · 2008 to 2008

$264k

NCI NIH HHS R01 CA100159

6 · The paper itself

Abstract

Elevated reactive oxidative species (ROS) are cytotoxic, and chronic elevated levels of ROS have been implicated in multiple diseases as well as cellular transformation and tumor progression. However, the potential for a transient and minimally toxic episode of ROS exposure, or a minimal threshold dose of ROS, to initiate disease or cellular transformation is unclear. We examined both transcriptional and phospho-proteomic responses of murine embryonic stem (ES) cells to a single brief exposure of minimally toxic hydrogen peroxide (H(2)O(2)). The cellular response was distinct from those induced by either an acute exposure to H(2)O(2) or the topoisomerase II poison etoposide. Analysis of tumorigenesis-related transcripts revealed a significant up-regulation of oncogenes and down-regulation of tumor suppressors. Analysis of the phospho-proteomic response demonstrated insulin-signaling induction, including insulin receptor Y972 hypophosphorylation, similar to insulin-resistance mouse models and observed in diabetic patients. In addition, ES cells were more resistant to ROS than differentiated cells, and retained their transcriptional self-renewal signature, suggesting stem cells have a higher potential for ROS-mediated mutagenesis and proliferation in vivo. These results are a direct demonstration that even brief and non-toxic exposures to ROS may induce transduction of insulin resistance and transformation signaling in stem cells leading to diabetes and cancer.

Identifiers

PMID21743783

PMCPMC3131088

OpenAlexW310812882

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.