ArticleInvestigative ophthalmology & visual science2011
Heparan sulfate regulates intraretinal axon pathfinding by retinal ganglion cells.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Signaling - transcription interactions in mouse retinal ganglion cells early axon pathfinding -a literature review.Frontiers in ophthalmology · 2023Review
- Flexible Roles for Proteoglycan Sulfation and Receptor Signaling.Trends in neurosciences · 2018Review
- A Comparison of Different Slicing Planes in Preservation of Major Hippocampal Pathway Fibers in the Mouse.Frontiers in neuroanatomy · 2017Article
- Functional Requirements for Heparan Sulfate Biosynthesis in Morphogenesis and Nervous System Development in C. elegans.PLoS genetics · 2017Article
- Panels of chemically-modified heparin polysaccharides and natural heparan sulfate saccharides both exhibit differences in binding to Slit and Robo, as well as variation between protein binding and cellular activity.Molecular bioSystems · 2016Article
- The role of heparan sulphate in development: the ectodermal story.International journal of experimental pathology · 2016Review
- Glypican Is a Modulator of Netrin-Mediated Axon Guidance.PLoS biology · 2015Article
- Macular optical coherence tomography in patients with unilateral optic nerve hypoplasia.Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus · 2015Article
- Retinal ganglion cell polarization using immobilized guidance cues on a tissue-engineered scaffold.Acta biomaterialia · 2014Article
- Role of heparan sulfate proteoglycans in optic disc and stalk morphogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Article
- Connecting the retina to the brain.ASN neuro · 2014Review
- Tissue engineering the retinal ganglion cell nerve fiber layer.Biomaterials · 2013Article
- Role of Filopodia in HSV-1 Entry into Zebrafish 3-O-Sulfotransferase-3-Expressing Cells.The open virology journal · 2013Article
- Article
- Anti-neurotrophic effects from autoantibodies in adult diabetes having primary open angle glaucoma or dementia.Frontiers in endocrinology · 2013Article
- The glomerular basement membrane as a model system to study the bioactivity of heparan sulfate glycosaminoglycans.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012Review
- BAC-Dkk3-EGFP transgenic mouse: an in vivo analytical tool for Dkk3 expression.Journal of biomedicine & biotechnology · 2012Article
- A roundabout way to cancer.Advances in cancer research · 2012Review
- Extracellular Matrix Components Regulate Cellular Polarity and Tissue Structure in the Developing and Mature Retina.Journal of ophthalmic & vision researchReview
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
PURPOSE. Heparan sulfate (HS) is abundantly expressed in the developing neural retina; however, its role in the intraretinal axon guidance of retinal ganglion cells (RGCs) remains unclear. In this study, the authors examined whether HS was essential for the axon guidance of RGCs toward the optic nerve head. METHODS. The authors conditionally ablated the gene encoding the exostosin-1 (Ext1) enzyme, using the dickkopf homolog 3 (Dkk3)-Cre transgene, which disrupted HS expression in the mouse retina during directed pathfinding by RGC axons toward the optic nerve head. In situ hybridization, immunohistochemistry, DiI tracing, binding assay, and retinal explant assays were performed to evaluate the phenotypes of the mutants and the roles of HS in intraretinal axon guidance. RESULTS. Despite no gross abnormality in RGC distribution, the mutant RGC axons exhibited severe intraretinal guidance errors, including optic nerve hypoplasia, ectopic axon penetration through the full thickness of the neural retina and into the subretinal space, and disturbance of the centrifugal projection of RGC axons toward the optic nerve head. These abnormal phenotypes shared similarities with the RGC axon misguidance caused by mutations of genes encoding Netrin-1 and Slit-1/2. Explant assays revealed that the mutant RGCs exhibited disturbed Netrin-1-dependent axon outgrowth and Slit-2-dependent repulsion. CONCLUSIONS. The present study demonstrated that RGC axon projection toward the optic nerve head requires the expression of HS in the neural retina, suggesting that HS in the retina functions as an essential modulator of Netrin-1 and Slit-mediated intraretinal RGC axon guidance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.