ArticleApplied and environmental microbiology2011
Genome data mining and soil survey for the novel group 5 [NiFe]-hydrogenase to explore the diversity and ecological importance of presumptive high-affinity H(2)-oxidizing bacteria.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Adaptations in electron transport chain complexes of clinical Rhodococcus equi revealed through comparative genomics.World journal of microbiology & biotechnology · 2026Article
- HApplied microbiology and biotechnology · 2026Article
- Frankia [NiFe] uptake hydrogenases and genome reduction: different lineages of loss.FEMS microbiology ecology · 2024Article
- Illegal dumping of oil and gas wastewater alters arid soil microbial communities.Applied and environmental microbiology · 2024Article
- Prevalence of trace gas-oxidizing soil bacteria increases with radial distance from Polloquere hot spring within a high-elevation Andean cold desert.The ISME journal · 2024Article
- Carbon amendments in soil microcosms induce uneven response on H2 oxidation activity and microbial community composition.FEMS microbiology ecology · 2023Article
- Atmospheric chemosynthesis is phylogenetically and geographically widespread and contributes significantly to carbon fixation throughout cold deserts.The ISME journal · 2022Article
- Environmental Adaptability and Organic Pollutant Degradation Capacity of a NovelMicroorganisms · 2022Article
- Microbial oxidation of atmospheric trace gases.Nature reviews. Microbiology · 2022Review
- Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system.Microbial cell factories · 2021Article
- Verrucomicrobial methanotrophs: ecophysiology of metabolically versatile acidophiles.FEMS microbiology reviews · 2021Review
- Acidobacteria are active and abundant members of diverse atmospheric HThe ISME journal · 2021Article
- Trace gas oxidizers are widespread and active members of soil microbial communities.Nature microbiology · 2021Article
- Genome-Based Characterization of Plant-AssociatedFrontiers in microbiology · 2021Article
- Hydrogen-Oxidizing Bacteria Are Abundant in Desert Soils and Strongly Stimulated by Hydration.mSystems · 2020Article
- A widely distributed hydrogenase oxidises atmospheric HThe ISME journal · 2020Article
- The rise of diversity in metabolic platforms across the Candidate Phyla Radiation.BMC biology · 2020Article
- The thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV oxidizes subatmospheric HThe ISME journal · 2020Article
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5 authors.
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Abstract
Streptomyces soil isolates exhibiting the unique ability to oxidize atmospheric H(2) possess genes specifying a putative high-affinity [NiFe]-hydrogenase. This study was undertaken to explore the taxonomic diversity and the ecological importance of this novel functional group. We propose to designate the genes encoding the small and large subunits of the putative high-affinity hydrogenase hhyS and hhyL, respectively. Genome data mining revealed that the hhyL gene is unevenly distributed in the phyla Actinobacteria, Proteobacteria, Chloroflexi, and Acidobacteria. The hhyL gene sequences comprised a phylogenetically distinct group, namely, the group 5 [NiFe]-hydrogenase genes. The presumptive high-affinity H(2)-oxidizing bacteria constituting group 5 were shown to possess a hydrogenase gene cluster, including the genes encoding auxiliary and structural components of the enzyme and four additional open reading frames (ORFs) of unknown function. A soil survey confirmed that both high-affinity H(2) oxidation activity and the hhyL gene are ubiquitous. A quantitative PCR assay revealed that soil contained 10(6) to 10(8) hhyL gene copies g (dry weight)(-1). Assuming one hhyL gene copy per genome, the abundance of presumptive high-affinity H(2)-oxidizing bacteria was higher than the maximal population size for which maintenance energy requirements would be fully supplied through the H(2) oxidation activity measured in soil. Our data indicate that the abundance of the hhyL gene should not be taken as a reliable proxy for the uptake of atmospheric H(2) by soil, because high-affinity H(2) oxidation is a facultatively mixotrophic metabolism, and microorganisms harboring a nonfunctional group 5 [NiFe]-hydrogenase may occur.
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