ArticleAnalytical biochemistry2011
Analysis of cholesteryl esters and diacylglycerols using lithiated adducts and electrospray ionization-tandem mass spectrometry.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Review
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- Data Processing Pipeline for Lipid Profiling of Carotid Atherosclerotic Plaque with Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2019Article
- Shotgun Analysis of Diacylglycerols Enabled by Thiol-ene Click Chemistry.Analytical chemistry · 2018Article
- Uncovering Structural Diversity of Unsaturated Fatty Acyls in Cholesteryl Esters via Photochemical Reaction and Tandem Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- PON3 knockout mice are susceptible to obesity, gallstone formation, and atherosclerosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Inhibiting monoacylglycerol acyltransferase 1 ameliorates hepatic metabolic abnormalities but not inflammation and injury in mice.The Journal of biological chemistry · 2014Article
- Development of an automated multi-injection shotgun lipidomics approach using a triple quadrupole mass spectrometer.Lipids · 2014Article
- Long-term Expression of Apolipoprotein B mRNA-specific Hammerhead Ribozyme via scAAV8.2 Vector Inhibits Atherosclerosis in Mice.Molecular therapy. Nucleic acids · 2013Article
- Quantitative profiling of major neutral lipid classes in human meibum by direct infusion electrospray ionization mass spectrometry.Investigative ophthalmology & visual science · 2013Article
- Cholesterol efflux analyses using stable isotopes and mass spectrometry.Analytical biochemistry · 2013Article
- Obesity-related alterations in cardiac lipid profile and nondipping blood pressure pattern during transition to diastolic dysfunction in male db/db mice.Endocrinology · 2013Article
- Gas phase chemistry of Li+ with amides: the observation of LiOH loss in mass spectrometry.Journal of the American Society for Mass Spectrometry · 2012Article
- Electrospray ionization tandem mass spectrometry of sodiated adducts of cholesteryl esters.Lipids · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cholesteryl ester (CE) and diacylglycerol (DAG) molecular species are important lipid storage and signaling molecules. Mass spectrometric analyses of these lipids are complicated by the presence of isobaric molecular ions shared by these lipid classes and by relatively poor electrospray ionization, which is a consequence of an inherently weak dipole moment in these lipid classes. The current study demonstrates that lithiated adducts of CE and DAG molecular ions have enhanced ionization and lipid class-specific fragmentation in tandem mass spectrometry (MS/MS) scan modes, thereby allowing the implementation of strategies capable of lipid class-specific detection. Using neutral loss (NL) mode for the loss of cholestane from cholesterol esters (NL 368.5) and specific selected reaction monitoring for DAG molecular species, the response of specific molecular species to that of internal standards was determined. CE and DAG molecular species were quantified in human coronary artery endothelial cells (HCAECs) incubated with both palmitic acid and oleic acid. Furthermore, NL 368.5 spectra revealed the oxidation of the aliphatic fatty acid residues of CE molecular species. Taken together, these studies demonstrate a new analytical approach to assessing CE and DAG molecular species that exploits the utility of lithiated adducts in conjunction with MS/MS approaches.
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