ArticleMolecular and cellular biology1990
Characterization of a thyroid-specific enhancer located 5.5 kilobase pairs upstream of the human thyroid peroxidase gene.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 71 citations in OpenAlex.
- A transgene targeted to the zebrafish nkx2.4b locus drives specific green fluorescent protein expression and disrupts thyroid development.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Murine hypothalamic destruction with vascular cell apoptosis subsequent to combined administration of human papilloma virus vaccine and pertussis toxin.Scientific reports · 2016Article
- Molecular cloning and functional characterization of two forms of Pax8 in the rainbow trout, Oncorhynchus mykiss.General and comparative endocrinology · 2014Article
- Thyroid regeneration: how stem cells play a role?Frontiers in endocrinology · 2014Review
- Stem cell antigen 1-positive mesenchymal cells are the origin of follicular cells during thyroid regeneration.PloS one · 2013Article
- Thyroid-specific transcription factors and their roles in thyroid cancer.Journal of thyroid research · 2011Article
- Role of NKX2-1 in N-bis(2-hydroxypropyl)-nitrosamine-induced thyroid adenoma in mice.Carcinogenesis · 2009Article
- Review: the role of neural crest cells in the endocrine system.Endocrine pathology · 2009Review
- Thyroid-specific enhancer-binding protein/NKX2.1 is required for the maintenance of ordered architecture and function of the differentiated thyroid.Molecular endocrinology (Baltimore, Md.) · 2006Article
- claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and stomach-specific isoforms through alternative splicing.Molecular and cellular biology · 2001Article
- Thyroid transcription factor 1 is calcium modulated and coordinately regulates genes involved in calcium homeostasis in C cells.Molecular and cellular biology · 1998Article
- Autoregulation of thyroid-specific gene transcription by thyroglobulin.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- PAX 8 activates the enhancer of the human thyroperoxidase gene.The Biochemical journal · 1998Article
- Cell-type-specific expression of the rat thyroperoxidase promoter indicates common mechanisms for thyroid-specific gene expression.Molecular and cellular biology · 1992Article
- Thyroid-specific enhancer-binding protein (T/EBP): cDNA cloning, functional characterization, and structural identity with thyroid transcription factor TTF-1.Molecular and cellular biology · 1991Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1991Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 6.3-kbp segment of DNA, upstream of the human thyroid peroxidase gene, and various deletions thereof were linked to a promoterless bacterial chloramphenicol acetyltransferase reporter gene. These constructs were analyzed by transfection and expression in rat FRTL-5 thyroid cells and in human hepatoma HepG2 cells to localize sequences that are important for thyroid cell-specific expression of the thyroid peroxidase gene. A thyroid-specific enhancer element, capable of activating enhancerless simian virus 40 promoter expression in FRTL-5 cells, was localized to a 230-bp region approximately 5.5 kbp upstream of the human thyroid peroxidase gene transcription start site. DNase I footprinting, using nuclear extracts prepared from FRTL-5 cells, revealed three regions within the 230-bp fragment; none of these regions were protected by nuclear extracts from HepG2 cells. Gel mobility shift assays, using double-stranded oligonucleotides corresponding to the three protected regions, further confirmed the existence of factors in FRTL-5 cells, but not HepG2 cells, able to specifically bind to the enhancer sequences. These results suggest the presence of three cis-acting DNA elements in the human thyroid peroxidase gene enhancer that interact with thyroid-specific trans-acting factors.
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