ArticleMolecular and cellular biochemistry1990
Phosphorylation of smooth muscle myosin by type II Ca2+/calmodulin-dependent protein kinase.
Article in Molecular and cellular biochemistry, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Differential control of calcium homeostasis and vascular reactivity by Ca2+/calmodulin-dependent kinase II.Hypertension (Dallas, Tex. : 1979) · 2013Article
- Structure of a highly conserved domain of Rock1 required for Shroom-mediated regulation of cell morphology.PloS one · 2013Article
- Smooth muscle signalling pathways in health and disease.Journal of cellular and molecular medicine · 2008Review
- Slow motility in hair cells of the frog amphibian papilla: myosin light chain-mediated shape change.Hearing research · 2008Article
- Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement.Cell & chromosome · 2007Article
- Integrin-linked kinase is responsible for Ca2+-independent myosin diphosphorylation and contraction of vascular smooth muscle.The Biochemical journal · 2005Article
- Differential functional properties of calmodulin-dependent protein kinase IIgamma variants isolated from smooth muscle.The Biochemical journal · 2003Article
- Characterization of Ca2+/calmodulin-dependent protein kinase I as a myosin II regulatory light chain kinase in vitro and in vivo.The Biochemical journal · 2002Article
- Neuronal cell shape and neurite initiation are regulated by the Ndr kinase SAX-1, a member of the Orb6/COT-1/warts serine/threonine kinase family.Molecular biology of the cell · 2000Article
- Ca2+-calmodulin-dependent protein kinase II-dependent activation of contractility in ferret aorta.The Journal of physiology · 2000Article
- Ca2+-independent phosphorylation of myosin in rat caudal artery and chicken gizzard myofilaments.The Journal of physiology · 1999Article
- alpha1-Adrenoceptor-mediated phosphorylation of myosin in rat-tail arterial smooth muscle.The Biochemical journal · 1997Article
- Calmodulin and the regulation of smooth muscle contraction.Molecular and cellular biochemistry · 1994Review
- Effects of calyculin A on tension and myosin phosphorylation in skinned smooth muscle of the rabbit mesenteric artery.British journal of pharmacology · 1993Article
- G protein-mediated inhibition of myosin light-chain phosphatase in vascular smooth muscle.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Phosphorylation of smooth muscle myosin by type II Ca2+/calmodulin-dependent protein kinase.Molecular and cellular biochemistry · 1990Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Brain type II Ca2+/calmodulin-dependent protein kinase was found to phosphorylate smooth muscle myosin, incorporating maximally approximately 2 mol of phosphoryl per mol of myosin, exclusively on the 20,000 dalton light chain subunit. After maximal phosphorylation of myosin or the isolated 20,000 dalton light chain subunit by myosin light chain kinase, the addition of type II Ca2+/calmodulin-dependent protein kinase led to no further incorporation indicating the two kinases phosphorylated a common site. This conclusion was supported by two dimensional mapping of tryptic digests of myosin phosphorylated by the two kinases. By phosphoamino acid analysis the phosphorylated residue was identified as a serine. The phosphorylation by type II Ca2+/calmodulin-dependent protein kinase of myosin resulted in enhancement of its actin-activated Mg2(+)-ATPase activity. Taken together, these data strongly support the conclusion that type II Ca2+/calmodulin-dependent protein kinase phosphorylates the same amino acid residue on the 20,000 dalton light chain subunit of smooth muscle myosin as is phosphorylated by myosin light chain kinase and suggest an alternative mechanism for the regulation of actin-myosin interaction.
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