ArticleDevelopmental biology2011
Boule-like genes regulate male and female gametogenesis in the flatworm Macrostomum lignano.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- Article
- Optimized protocols for RNA interference in Macrostomum lignano.G3 (Bethesda, Md.) · 2024Article
- Article
- Single-cell analysis of Schistosoma mansoni identifies a conserved genetic program controlling germline stem cell fate.Nature communications · 2021Article
- Lost and Found: Piwi and Argonaute Pathways in Flatworms.Frontiers in cellular and infection microbiology · 2021Article
- De novo transcriptome assembly from the gonads of a scleractinian coral, Euphyllia ancora: molecular mechanisms underlying scleractinian gametogenesis.BMC genomics · 2020Article
- The free-living flatwormEvoDevo · 2020Review
- Seminal fluid-mediated fitness effects in the simultaneously hermaphroditic flatwormEcology and evolution · 2019Article
- Polymorphisms within theAnimals : an open access journal from MDPI · 2019Article
- A mechanism for temporary bioadhesion.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A targeted in situ hybridization screen identifies putative seminal fluid proteins in a simultaneously hermaphroditic flatworm.BMC evolutionary biology · 2018Article
- Genome-wide transcriptome profiling and spatial expression analyses identify signals and switches of development in tapeworms.EvoDevo · 2018Article
- The Conservation of the Germline Multipotency Program, from Sponges to Vertebrates: A Stepping Stone to Understanding the Somatic and Germline Origins.Genome biology and evolution · 2017Article
- Article
- A premeiotic function for boule in the planarian Schmidtea mediterranea.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Adhesive organ regeneration in Macrostomum lignano.BMC developmental biology · 2016Article
- Sperm competition-induced plasticity in the speed of spermatogenesis.BMC evolutionary biology · 2016Article
- Basal bodies across eukaryotes series: basal bodies in the freshwater planarian Schmidtea mediterranea.Cilia · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the DAZ (Deleted in AZoospermia) gene family are important players in the process of gametogenesis and their dysregulation accounts for 10% of human male infertility. Boule, the ancestor of the family, is mainly involved in male meiosis in most organisms. With the exception of Drosophila and C. elegans, nothing is known on the function of boule in non-vertebrate animals. In the present study, we report on three boule orthologues in the flatworm Macrostomum lignano. We demonstrate that macbol1 and macbol2 are expressed in testes whilst macbol3 is expressed in ovaries and developing eggs. Macbol1 RNAi blocked spermatocyte differentiation whereas macbol2 showed no effect upon RNAi treatment. Macbol3 RNAi resulted in aberrant egg maturation and led to female sterility. We further demonstrated the evolutionary functional conservation of macbol1 by introducing this gene into Drosophila bol(1) mutants. Macbol1 was able to rescue the progression of fly meiotic divisions. In summary, our findings provide evidence for an involvement of boule genes in male and female gamete development in one organism. Furthermore, boule gene function is shown here for the first time in a lophotrochozoan. Our results point to a more diverse functional assignment of boule genes. Therefore, a better understanding of boule function in flatworms can help to elucidate the molecular mechanisms of and concomitant infertility in higher organisms including humans.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.