ArticleProtein science : a publication of the Protein Society2011
Computational design and selections for an engineered, thermostable terpene synthase.
Article in Protein science : a publication of the Protein Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Advances in the sustainable biosynthesis of valuable terpenoid flavor compounds and precursors in micro-organisms.Biotechnology for biofuels and bioproducts · 2025Review
- Rapid Screening of Plastic-Degrading Enzymes Using an Optimized Cell-Free Protein Synthesis Platform.Journal of microbiology and biotechnology · 2025Article
- Engineering terpene synthases and their substrates for the biocatalytic production of terpene natural products and analogues.Chemical communications (Cambridge, England) · 2025Review
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
- Decoding Catalysis by Terpene Synthases.ACS catalysis · 2023Review
- Genetically Encoded Detection of Biosynthetic Protease Inhibitors.ACS synthetic biology · 2023Article
- Single-molecule Taq DNA polymerase dynamics.Science advances · 2022Article
- Empirical comparison and analysis of machine learning-based predictors for predicting and analyzing of thermophilic proteins.EXCLI journal · 2022Review
- A novel sequence-based predictor for identifying and characterizing thermophilic proteins using estimated propensity scores of dipeptides.Scientific reports · 2021Article
- Rerouting and Improving Dauc-8-en-11-ol Synthase from Streptomyces venezuelae to a High Yielding Biocatalyst.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Stabilizing AqdC, a Pseudomonas Quinolone Signal-Cleaving Dioxygenase from Mycobacteria, by FRESCO-Based Protein Engineering.Chembiochem : a European journal of chemical biology · 2021Article
- Germacrene A-A Central Intermediate in Sesquiterpene Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Protein engineering strategies for microbial production of isoprenoids.Metabolic engineering communications · 2020Review
- Manipulation of the precursor supply for high-level production of longifolene by metabolically engineered Escherichia coli.Scientific reports · 2019Article
- Catalysis of amorpha-4,11-diene synthase unraveled and improved by mutability landscape guided engineering.Scientific reports · 2018Article
- Review: Engineering of thermostable enzymes for industrial applications.APL bioengineering · 2018Review
- Structural and Chemical Biology of Terpenoid Cyclases.Chemical reviews · 2017Review
- Characterization of the first naturally thermostable terpene synthases and development of strategies to improve thermostability in this family of enzymes.The FEBS journal · 2017Article
- Ten-Minute Protein Purification and Surface Tethering for Continuous-Flow Biocatalysis.Angewandte Chemie (International ed. in English) · 2017Article
- Biosynthetic potential of sesquiterpene synthases: product profiles of Egyptian Henbane premnaspirodiene synthase and related mutants.The Journal of antibiotics · 2016Article
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Authors and funding
9 authors.
Funding
Abstract
Terpenoids include structurally diverse antibiotics, flavorings, and fragrances. Engineering terpene synthases for control over the synthesis of such compounds represents a long sought goal. We report computational design, selections, and assays of a thermostable mutant of tobacco 5-epi-aristolochene synthase (TEAS) for the catalysis of carbocation cyclization reactions at elevated temperatures. Selection for thermostability included proteolytic digestion followed by capture of intact proteins. Unlike the wild-type enzyme, the mutant TEAS retains enzymatic activity at 65°C. The thermostable terpene synthase variant denatures above 80°C, approximately twice the temperature of the wild-type enzyme.
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