ArticlePloS one2011
X chromosome inactivation and differentiation occur readily in ES cells doubly-deficient for macroH2A1 and macroH2A2.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- MacroH2A2-Enriched Domains Are Largely Stable Across the Cell Cycle but Focally Displaced at Mitotic Regulatory Elements.International journal of molecular sciences · 2026Article
- Regional Conservation and Transcriptional Regulation of Tumor-Associated Genes by macroH2A1 Deposition in Mammalian Cells.Biomolecules · 2025Article
- Xist condensates: perspectives for therapeutic intervention.Genome biology · 2025Review
- Article
- Epigenetic Regulation of Myogenesis: Focus on the Histone Variants.International journal of molecular sciences · 2021Review
- The Multiple Facets of ATRX Protein.Cancers · 2021Review
- The roles of histone variants in fine-tuning chromatin organization and function.Nature reviews. Molecular cell biology · 2020Review
- Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination.Nature communications · 2020Article
- Deficiency of 15-LOX-1 Induces Radioresistance through Downregulation of MacroH2A2 in Colorectal Cancer.Cancers · 2019Article
- Zebrafish macroH2A variants have distinct embryo localization and function.Scientific reports · 2019Article
- Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains.The Journal of cell biology · 2019Article
- Progress in understanding the molecular mechanism ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- A standardized human embryoid body platform for the detection and analysis of teratogens.PloS one · 2017Article
- Loss of histone variant macroH2A2 expression associates with progression of anal neoplasm.Journal of clinical pathology · 2016Article
- MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B.Oncogene · 2016Article
- Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST.Genome biology · 2015Article
- Histone variants and cellular plasticity.Trends in genetics : TIG · 2015Review
- Mice without macroH2A histone variants.Molecular and cellular biology · 2014Article
- Histone variants: emerging players in cancer biology.Cellular and molecular life sciences : CMLS · 2014Review
- Long nonoding RNAs in the X-inactivation center.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Macrohistones (mH2As) are unusual histone variants found exclusively in vertebrate chromatin. In mice, the H2afy gene encodes two splice variants, mH2A1.1 and mH2A1.2 and a second gene, H2afy2, encodes an additional mH2A2 protein. Both mH2A isoforms have been found enriched on the inactive X chromosome (Xi) in differentiated mammalian female cells, and are incorporated into the chromatin of developmentally-regulated genes. To investigate the functional significance of mH2A isoforms for X chromosome inactivation (XCI), we produced male and female embryonic stem cell (ESC) lines with stably-integrated shRNA constructs that simultaneously target both mH2A1 and mH2A2. Surprisingly, we find that female ESCs deficient for both mH2A1 and mH2A2 readily execute and maintain XCI upon differentiation. Furthermore, male and female mH2A-deficient ESCs proliferate normally under pluripotency culture conditions, and respond to several standard differentiation procedures efficiently. Our results show that XCI can readily proceed with substantially reduced total mH2A content.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.