ArticlePloS one2011
Rapid analysis of Listeria monocytogenes cell wall teichoic acid carbohydrates by ESI-MS/MS.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Isolation and relationship analysis of Listeria phages with various serotype hosts and morphological characterization.Virology journal · 2025Article
- Deletion of glycosyltransferaseVirulence · 2024Article
- A CRISPR interference screen reveals a role for cell wall teichoic acids in conjugation in Bacillus subtilis.Molecular microbiology · 2022Article
- Analysis of Derivatized Wall Teichoic Acids Confirms that a Mutation in Phage-ResistantACS omega · 2022Article
- Glycotyping and Specific Separation of Listeria monocytogenes with a Novel Bacteriophage Protein Tool Kit.Applied and environmental microbiology · 2020Article
- Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.Molecular microbiology · 2020Article
- Characterization of Listeria prophages in lysogenic isolates from foods and food processing environments.PloS one · 2019Article
- Extraction and Analysis of Bacterial Teichoic Acids.Bio-protocol · 2018Article
- Molecular Basis of Bacterial Host Interactions by Gram-Positive Targeting Bacteriophages.Viruses · 2018Review
- Salt-Induced Stress Stimulates a Lipoteichoic Acid-Specific Three-Component Glycosylation System in Staphylococcus aureus.Journal of bacteriology · 2018Article
- Structural and functional diversity inThe Journal of biological chemistry · 2017Article
- A new approach to decoupling of bacterial adhesion energies measured by AFM into specific and nonspecific components.Colloid and polymer science · 2014Article
- (D)-Amino acid chemical reporters reveal peptidoglycan dynamics of an intracellular pathogen.ACS chemical biology · 2013Article
- Wall teichoic acids restrict access of bacteriophage endolysin Ply118, Ply511, and PlyP40 cell wall binding domains to the Listeria monocytogenes peptidoglycan.Journal of bacteriology · 2012Article
- Integrative genomic analysis identifies isoleucine and CodY as regulators of Listeria monocytogenes virulence.PLoS genetics · 2012Article
- Listeria monocytogenes tyrosine phosphatases affect wall teichoic acid composition and phage resistance.FEMS microbiology letters · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the application of electrospray ionization (ESI) mass spectrometry for compositional characterization of wall teichoic acids (WTA), a major component of gram-positive bacterial cell walls. Tandem mass spectrometry (ESI-MS/MS) of purified and chemically hydrolyzed monomeric WTA components provided sufficient information to identify WTA monomers and their specific carbohydrate constituents. A lithium matrix was used for ionization of uncharged WTA monomers, and successfully applied to analyze the WTA molecules of four Listeria strains differing in carbohydrate substitution on a conserved polyribitol-phosphate backbone structure. Carbohydrate residues such as N-acetylglucosamine or rhamnose linked to the WTA could directly be identified by ESI-MS/MS, circumventing the need for quantitative analysis by gas chromatography. The presence of a terminal N-acetylglucosamine residue tethered to the ribitol was confirmed using fluorescently labeled wheat-germ agglutinin. In conclusion, the mass spectrometry method described here will greatly facilitate compositional analysis and characterization of teichoic acids and similar macromolecules from diverse bacterial species, and represents a significant advance in the identification of serovar-specific carbohydrates and sugar molecules on bacteria.
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