ArticlePLoS genetics2011
Independent chromatin binding of ARGONAUTE4 and SPT5L/KTF1 mediates transcriptional gene silencing.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 76 citations in OpenAlex.
- Molecular Mechanisms Underlying the Establishment, Maintenance, and Removal of DNA Methylation in Plants.Annual review of plant biology · 2025Review
- Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducingGenome research · 2025Article
- A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis.Nature communications · 2024Article
- A newly identified glycosyltransferase AsRCOM provides resistance to purple curl leaf disease in agave.BMC genomics · 2023Article
- Phylogenetic analyses of seven protein families refine the evolution of small RNA pathways in green plants.Plant physiology · 2023Article
- Genome-Wide Identification and Posttranscriptional Regulation Analyses Elucidate Roles of Key Argonautes and Their miRNA Triggers in Regulating Complex Yield Traits in Rapeseed.International journal of molecular sciences · 2023Article
- Identification of loci controlling timing of stem elongation in red clover using genotyping by sequencing of pooled phenotypic extremes.Molecular genetics and genomics : MGG · 2022Article
- The transient Spt4-Spt5 complex as an upstream regulator of non-coding RNAs during development.Nucleic acids research · 2022Article
- An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation.Nature plants · 2021Article
- The immune repressor BIR1 contributes to antiviral defense and undergoes transcriptional and post-transcriptional regulation during viral infections.The New phytologist · 2019Article
- RNA-directed DNA methylation involves co-transcriptional small-RNA-guided slicing of polymerase V transcripts in Arabidopsis.Nature plants · 2018Article
- Structural disorder in plant proteins: where plasticity meets sessility.Cellular and molecular life sciences : CMLS · 2017Review
- Functional Dissection of the Pol V Largest Subunit CTD in RNA-Directed DNA Methylation.Cell reports · 2017Article
- A meiosis-specific Spt5 homolog involved in non-coding transcription.Nucleic acids research · 2017Article
- Long-range control of gene expression via RNA-directed DNA methylation.PLoS genetics · 2017Article
- The RNAs of RNA-directed DNA methylation.Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- Global identification and expression analysis of stress-responsive genes of the Argonaute family in apple.Molecular genetics and genomics : MGG · 2016Article
- A Multigenic Network of ARGONAUTE4 Clade Members Controls Early Megaspore Formation in Arabidopsis.Genetics · 2016Article
- Two Components of the RNA-Directed DNA Methylation Pathway Associate with MORC6 and Silence Loci Targeted by MORC6 in Arabidopsis.PLoS genetics · 2016Article
- A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis.Molecular cell · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic genomes contain significant amounts of transposons and repetitive DNA elements, which, if transcribed, can be detrimental to the organism. Expression of these elements is suppressed by establishment of repressive chromatin modifications. In Arabidopsis thaliana, they are silenced by the siRNA-mediated transcriptional gene silencing pathway where long non-coding RNAs (lncRNAs) produced by RNA Polymerase V (Pol V) guide ARGONAUTE4 (AGO4) to chromatin and attract enzymes that establish repressive chromatin modifications. It is unknown how chromatin modifying enzymes are recruited to chromatin. We show through chromatin immunoprecipitation (ChIP) that SPT5L/KTF1, a silencing factor and a homolog of SPT5 elongation factors, binds chromatin at loci subject to transcriptional silencing. Chromatin binding of SPT5L/KTF1 occurs downstream of RNA Polymerase V, but independently from the presence of 24-nt siRNA. We also show that SPT5L/KTF1 and AGO4 are recruited to chromatin in parallel and independently of each other. As shown using methylation-sensitive restriction enzymes, binding of both AGO4 and SPT5L/KTF1 is required for DNA methylation and repressive histone modifications of several loci. We propose that the coordinate binding of SPT5L and AGO4 creates a platform for direct or indirect recruitment of chromatin modifying enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.