ArticleMolecular biology of the cell2011
Translocation channel gating kinetics balances protein translocation efficiency with signal sequence recognition fidelity.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Advances in SEC61G research: from ER translocon subunit to emerging pan-cancer oncogenic roles.Cancer biology & therapy · 2026Review
- A unifying model for membrane protein biogenesis.Nature structural & molecular biology · 2024Review
- Signal Peptide Features Determining the Substrate Specificities of Targeting and Translocation Components in Human ER Protein Import.Frontiers in physiology · 2022Review
- Folding and Insertion of Transmembrane Helices at the ER.International journal of molecular sciences · 2021Review
- Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B inmicroPublication biology · 2021Article
- Complexity and Specificity of Sec61-Channelopathies: Human Diseases Affecting Gating of the Sec61 Complex.Cells · 2021Review
- A ribosome-associated chaperone enables substrate triage in a cotranslational protein targeting complex.Nature communications · 2020Article
- Understanding the mammalian TRAP complex function(s).Open biology · 2020Review
- Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum inMolecular biology of the cell · 2019Article
- The signal peptide plus a cluster of positive charges in prion protein dictate chaperone-mediated Sec61 channel gating.Biology open · 2019Article
- Conserved motifs on the cytoplasmic face of the protein translocation channel are critical for the transition between resting and active conformations.The Journal of biological chemistry · 2018Article
- A signal sequence suppressor mutant that stabilizes an assembled state of the twin arginine translocase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- BiP clustering facilitates protein folding in the endoplasmic reticulum.PLoS computational biology · 2014Article
- Structural and functional profiling of the lateral gate of the Sec61 translocon.The Journal of biological chemistry · 2014Article
- Article
- ERAD and protein import defects in a sec61 mutant lacking ER-lumenal loop 7.BMC cell biology · 2013Article
- Protein translocation across the rough endoplasmic reticulum.Cold Spring Harbor perspectives in biology · 2013Review
- A gating motif in the translocation channel sets the hydrophobicity threshold for signal sequence function.The Journal of cell biology · 2012Article
- Direct simulation of early-stage Sec-facilitated protein translocation.Journal of the American Chemical Society · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The transition between the closed and open conformations of the Sec61 complex permits nascent protein insertion into the translocation channel. A critical event in this structural transition is the opening of the lateral translocon gate that is formed by four transmembrane (TM) spans (TM2, TM3, TM7, and TM8 in Sec61p) to expose the signal sequence-binding site. To gain mechanistic insight into lateral gate opening, mutations were introduced into a lumenal loop (L7) that connects TM7 and TM8. The sec61 L7 mutants were found to have defects in both the posttranslational and cotranslational translocation pathways due to a kinetic delay in channel gating. The translocation defect caused by L7 mutations could be suppressed by the prl class of sec61 alleles, which reduce the fidelity of signal sequence recognition. The prl mutants are proposed to act by destabilizing the closed conformation of the translocation channel. Our results indicate that the equilibrium between the open and closed conformations of the protein translocation channel maintains a balance between translocation activity and signal sequence recognition fidelity.
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