ArticleMolecular biology of the cell2011
Microtubule assembly in meiotic extract requires glycogen.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- Glycogen-dependent demixing of frog egg cytoplasm at increased crowding.Biophysical journal · 2026Article
- Neuronal glycogen breakdown mitigates tauopathy via pentose-phosphate-pathway-mediated oxidative stress reduction.Nature metabolism · 2025Article
- Identification of glycogen phosphorylase L as a potential target for lung cancer.Medical oncology (Northwood, London, England) · 2023Article
- Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly inMolecular biology of the cell · 2022Article
- Article
- Affinity Purification of Label-free Tubulins fromSTAR protocols · 2020Article
- A computational model of the early stages of acentriolar meiotic spindle assembly.Molecular biology of the cell · 2019Article
- Proteomic Profiling of Microtubule Self-organization in M-phase.Molecular & cellular proteomics : MCP · 2018Article
- Controlling compartmentalization by non-membrane-bound organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Glycogen synthase protects neurons from cytotoxicity of mutant huntingtin by enhancing the autophagy flux.Cell death & disease · 2018Article
- Chromosomal passenger complex hydrodynamics suggests chaperoning of the inactive state by nucleoplasmin/nucleophosmin.Molecular biology of the cell · 2017Article
- Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol.Molecular biology of the cell · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The assembly of microtubules during mitosis requires many identified components, such as γ-tubulin ring complex (γ-TuRC), components of the Ran pathway (e.g., TPX2, HuRP, and Rae1), and XMAP215/chTOG. However, it is far from clear how these factors function together or whether more factors exist. In this study, we used biochemistry to attempt to identify active microtubule nucleation protein complexes from Xenopus meiotic egg extracts. Unexpectedly, we found both microtubule assembly and bipolar spindle assembly required glycogen, which acted both as a crowding agent and as metabolic source glucose. By also reconstituting microtubule assembly in clarified extracts, we showed microtubule assembly does not require ribosomes, mitochondria, or membranes. Our clarified extracts will provide a powerful tool for activity-based biochemical fractionations for microtubule assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.