ArticleThe Journal of clinical investigation1990
Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan.
Article in The Journal of clinical investigation, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 124 citations in OpenAlex.
- Changes in arachidonic acid (AA)- and linoleic acid (LA)-derived hydroxy metabolites and their interplay with inflammatory biomarkers in response to drastic changes in air pollution exposure.Environmental research · 2021Article
- Metabolic reprograming of LPS-stimulated human lung macrophages involves tryptophan metabolism and the aspartate-arginosuccinate shunt.PloS one · 2020Article
- Novel AU-rich proximal UTR sequences (APS) enhance CXCL8 synthesis upon the induction of rpS6 phosphorylation.PLoS genetics · 2019Article
- Fatty acid binding protein 4/aP2-dependent BLT1R expression and signaling.Journal of lipid research · 2017Article
- Purified and Recombinant Hemopexin: Protease Activity and Effect on Neutrophil Chemotaxis.Molecular medicine (Cambridge, Mass.) · 2016Article
- G protein-coupled receptors as targets for anti-diabetic therapeutics.Nature reviews. Drug discovery · 2016Review
- Purification of a lectin from Arisaema erubescens (Wall.) Schott and its pro-inflammatory effects.Molecules (Basel, Switzerland) · 2011Article
- Neutrophilic airways inflammation in lung cancer: the role of exhaled LTB-4 and IL-8.BMC cancer · 2011Article
- A distinctive role of the leukotriene B4 receptor BLT1 in osteoclastic activity during bone loss.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- LTB4 is present in exudative pleural effusions and contributes actively to neutrophil recruitment in the inflamed pleural space.Clinical and experimental immunology · 2004Article
- Modulation of plasma levels of soluble adhesion molecules and nitric oxide in healthy volunteers by exposure to swine dust.Inflammation · 2002Article
- Increased levels of interleukin-8 in BAL fluid from smokers susceptible to pulmonary emphysema.Thorax · 2002Article
- Tumour necrosis factor-alpha and leukotriene B(4) mediate the neutrophil migration in immune inflammation.British journal of pharmacology · 2001Article
- Pathogenesis of porcine Actinobacillus pleuropneumonia, part II: roles of proinflammatory cytokines.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
- Evaluation of inflammatory cytokine secretion by human alveolar macrophages.Mediators of inflammation · 1999Article
- Synergistic effects of bovine respiratory syncytial virus and non-cytopathic bovine viral diarrhea virus infection on selected bovine alveolar macrophage functions.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
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- Role of pertussis toxin A subunit in neutrophil migration and vascular permeability.Infection and immunity · 1997Article
- Serotype-dependent induction of pulmonary neutrophilia and inflammatory cytokine gene expression by reovirus.Journal of virology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The capacity of lipopolysaccharide (LPS), zymosan, and calcium ionophore A23187 to induce neutrophil chemotactic activity (NCA), leukotriene B4 (LTB4), and neutrophil attractant/activation protein (NAP-1) release from human alveolar macrophages (AM) retrieved from normal nonsmokers was evaluated. LPS induced a dose-dependent release of LTB4 that began by 1 h, 4.0 +/- 3.2 ng/10(6) viable AM; peaked at 3 h, 24.7 +/- 13.5 ng/10(6) viable AM; and decreased by 24 h, 1.2 +/- 1.0 ng/10(6) viable AM (n = 8). Quantities of LTB4 in cell-free supernatants of AM stimulated with LPS were determined by reverse-phase high-performance liquid chromatography and corresponded well with results obtained by radioimmunoassay. By contrast, NAP-1 release began approximately 3-5 h after stimulation of AM with LPS, 197 +/- 192 ng/ml, and peaked at 24 h, 790 +/- 124 ng/ml. Release of NAP-1 was stimulus specific because A23187 evoked the release of LTB4 but not NAP-1, whereas LPS and zymosan induced the release of both LTB4 and NAP-1. The appearance of neutrophil chemotactic activity in supernatants of AM challenged with LPS for 3 h could be explained completely by the quantities of LTB4 present. After stimulation with LPS or zymosan for 24 h, AM had metabolized almost all generated LTB4. Preincubation of AM with nordihydroguiaretic acid (10(-4) M) completely abolished the appearance of NCA, LTB4, and NAP-1 in supernatants of AM challenged with LPS. Therefore, LPS and zymosan particles were potent stimuli of the sequential release of LTB4 and NAP-1 from AM.
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