ArticleTraffic (Copenhagen, Denmark)2011
Recruitment of cellular clathrin to viral factories and disruption of clathrin-dependent trafficking.
Article in Traffic (Copenhagen, Denmark), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs.Nature communications · 2023Article
- Mammalian orthoreovirus core protein μ2 reorganizes host microtubule-organizing center components.Virology · 2020Article
- The roles of grouper clathrin light chains in regulating the infection of a novel marine DNA virus, Singapore grouper iridovirus.Scientific reports · 2019Article
- Characterization of a Replicating Mammalian Orthoreovirus with Tetracysteine-Tagged μNS for Live-Cell Visualization of Viral Factories.Journal of virology · 2017Article
- Reovirus inhibits interferon production by sequestering IRF3 into viral factories.Scientific reports · 2017Article
- Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin.Traffic (Copenhagen, Denmark) · 2017Article
- Identification and characterization of two cleavage fragments from the Aquareovirus nonstructural protein NS80.Virologica Sinica · 2016Article
- The non-structural protein μNS of piscine orthoreovirus (PRV) forms viral factory-like structures.Veterinary research · 2016Article
- Clathrin mediates infectious hepatitis C virus particle egress.Journal of virology · 2015Article
- Proteolytic disassembly of viral outer capsid proteins is crucial for reovirus-mediated type-I interferon induction in both reovirus-susceptible and reovirus-refractory tumor cells.BioMed research international · 2015Article
- Engineering recombinant reoviruses with tandem repeats and a tetravirus 2A-like element for exogenous polypeptide expression.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Article
- Nonstructural protein NS80 is crucial in recruiting viral components to form aquareoviral factories.PloS one · 2013Article
- Reovirus replication protein μ2 influences cell tropism by promoting particle assembly within viral inclusions.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The viral factories of mammalian reovirus (MRV) are cytoplasmic structures that serve as sites of viral genome replication and particle assembly. A 721-aa MRV non-structural protein, µNS, forms the factory matrix and recruits other viral proteins to these structures. In this report, we show that µNS contains a conserved C-proximal sequence (711-LIDFS-715) that is similar to known clathrin-box motifs and is required for recruitment of clathrin to viral factories. Clathrin recruitment by µNS occurs independently of infecting MRV particles or other MRV proteins. Ala substitution for a single Leu residue (mutation L711A) within the putative clathrin-binding motif of µNS inhibits clathrin recruitment, but does not prevent formation or expansion of viral factories. Notably, clathrin-dependent cellular functions, including both endocytosis and secretion, are disrupted in cells infected with MRV expressing wild-type, but not L711A, µNS. These results identify µNS as a novel adaptor-like protein that recruits cellular clathrin to viral factories, disrupting normal functions of clathrin in cellular membrane trafficking. To our knowledge, this is the only viral or bacterial protein yet shown to interfere with clathrin functions in this manner. The results additionally establish a new approach for studies of clathrin functions, based on µNS-mediated sequestration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.