ArticleMarine biotechnology (New York, N.Y.)2012
Induction of mitogynogenetic diploids and identification of WW super-female using sex-specific SSR markers in half-smooth tongue sole (Cynoglossus semilaevis).
Article in Marine biotechnology (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 117 citations in OpenAlex.
- Potential Physiological and Molecular Mechanisms Underlying Low Viability of Gynogenetic WW-Type Super-Female Sterlet (International journal of molecular sciences · 2025Article
- Functional Study of Four Histone Genes Involved in the Spermatogenesis ofAnimals : an open access journal from MDPI · 2025Article
- Characterization of the sex determining region and development of a molecular sex identification method in a Salangid fish.BMC genomics · 2024Article
- Advances in biotechnology and breeding innovations in China's marine aquaculture.Advanced biotechnology · 2024Review
- Genome-Wide Association Studies (GWAS) and Transcriptome Analysis Reveal Male Heterogametic Sex-Determining Regions and Candidate Genes in Northern Snakeheads (International journal of molecular sciences · 2024Article
- DNA Methylation Mediates Sperm Quality viaInternational journal of molecular sciences · 2024Article
- Gender Impacted Gut Microbiota and Growth Performance in the Blotched Snakehead (Microorganisms · 2024Article
- Identification and Functional Analysis ofAnimals : an open access journal from MDPI · 2024Article
- Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus.GigaScience · 2024Article
- Phosphoproteomics Reveal New Candidates in Abnormal Spermatogenesis of Pseudomales inInternational journal of molecular sciences · 2023Article
- Identification and Expression Pattern ofAnimals : an open access journal from MDPI · 2022Article
- Microsatellite Characteristics of Silver Carp (Genes · 2022Article
- Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (International journal of molecular sciences · 2022Article
- Comparative piRNAs Profiles Give a Clue to Transgenerational Inheritance of Sex-Biased piRNAs in Cynoglossus semilaevis.Marine biotechnology (New York, N.Y.) · 2022Article
- Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius).BMC genomics · 2021Article
- A simple and rapid method for fish sex identification based on recombinase-aided amplification and its use in Cynoglossus semilaevis.Scientific reports · 2021Article
- Transcriptome Profiling Reveals the Sexual Dimorphism of Gene Expression Patterns during Gonad Differentiation in the Half-Smooth Tongue Sole (Cynoglossus semilaevis).Marine biotechnology (New York, N.Y.) · 2021Article
- Isolation of a Male-Specific Molecular Marker and Development of a Genetic Sex Identification Technique in Spotted Knifejaw (Oplegnathus punctatus).Marine biotechnology (New York, N.Y.) · 2020Article
- Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis.BMC genomics · 2020Article
- Comparative Genomics Studies on theFrontiers in genetics · 2020Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The half-smooth tongue sole (Cynoglossus semilaevis) is an important cultured marine fish as well as a promising model fish for the study of sex determination mechanisms. In the present study, a protocol for artificial induction of mitogynogenesis by hydrostatic pressure using heterologous sperm was developed in half-smooth tongue sole in order to assess homozygosity of gynogens and to identify WW super-female. The optimal initiation time for pressure shock of mitogynogenetic embryos was determined to be 21.5 min after insemination when water temperature is at 22-23°C, while the optimal pressure and treatment duration were determined to be 70 MPa for 4 min. About 1,500 mitogynogenetic diploid larvae were obtained. Ten tongue sole microsatellite markers were used for homozygosity analysis of 24 mitogynogenetic larvae. Among the 24 larvae, the percentage of homozygosity ranged from 73.91% to 87.50% with an average homozygosity of 80.54%. Sex-specific simple sequence repeat (SSR) markers, CseF-SSR1, were isolated and used for identifying WW super-female mitogynogens in the tongue sole. The amplification of genomic DNA using the sex-specific SSR marker produced one DNA band of 206 bp in ZZ males, two DNA bands of 206 and 218 bp in ZW females, and one DNA band of 218 bp in WW super-females. Four WW "super-female" gynogens were observed in 39 mitogynogenetic diploids, indicating a ZW sex determination mechanism in the tongue sole. Thus, a protocol for the induction of artificial mitogynogenesis has been developed for the first time in half-smooth tongue sole, and the WW super-female diploids were identified in the mitogynogens by sex-specific SSR markers. These findings lay the foundation and provide important tool for the elaboration of sex determination mechanism, generation of WW super-females, and development of clone line and breeding of all-female stock in the half-smooth tongue sole.
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