ArticleJournal of molecular medicine (Berlin, Germany)2011
Design and validation of an endothelial progenitor cell capture chip and its application in patients with pulmonary arterial hypertension.
Article in Journal of molecular medicine (Berlin, Germany), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Stem cell therapy in pulmonary hypertension: current practice and future opportunities.European respiratory review : an official journal of the European Respiratory Society · 2023Review
- Organ Chips and Visualization of Biological Systems.Advances in experimental medicine and biology · 2023Article
- Endothelial progenitor cell mobilization based on exercise volume in patients with cardiovascular disease and healthy individuals: a systematic review and meta-analysis.European heart journal open · 2022Review
- Therapeutic Potential of Endothelial Progenitor Cells in Pulmonary Diseases.American journal of respiratory cell and molecular biology · 2021Review
- Review
- Evidence of Accumulated Endothelial Progenitor Cells in the Lungs of Rats with Pulmonary Arterial Hypertension byMolecular therapy. Methods & clinical development · 2020Article
- Review
- Understanding angiogenesis during aging: opportunities for discoveries and new models.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Review
- Lab-on-a-Chip Platforms for Detection of Cardiovascular Disease and Cancer Biomarkers.Sensors (Basel, Switzerland) · 2017Review
- Circulating Endothelial Cell Quantification by Microfluidics Chip in Pulmonary Arterial Hypertension.American journal of respiratory cell and molecular biology · 2017Article
- Micro- and nanodevices integrated with biomolecular probes.Biotechnology advances · 2015Review
- Microfluidic capture of endothelial colony-forming cells from human adult peripheral blood: phenotypic and functional validation in vivo.Tissue engineering. Part C, Methods · 2015Article
- Contrasting biofunctionalization strategies for the enhanced endothelialization of biodegradable vascular grafts.Biomacromolecules · 2015Article
- Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.Reports on progress in physics. Physical Society (Great Britain) · 2015Review
- Pathway analysis and transcriptomics improve protein identification by shotgun proteomics from samples comprising small number of cells--a benchmarking study.BMC genomics · 2014Article
- Circulating endothelial and progenitor cells: Evidence from acute and long-term exercise effects.World journal of cardiology · 2012Article
- New models of pulmonary hypertension based on VEGF receptor blockade-induced endothelial cell apoptosis.Pulmonary circulation · 2012Article
- Generation of tissue constructs for cardiovascular regenerative medicine: from cell procurement to scaffold design.Biotechnology advancesReview
- Identification of functional progenitor cells in the pulmonary vasculature.Pulmonary circulationArticle
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
The number of circulating endothelial progenitor cells (EPCs) inversely correlates with cardiovascular risk and clinical outcome, and thus has been proposed as a valuable biomarker for risk assessment, disease progression, and response to therapy. However, current strategies for isolation of these rare cells are limited to complex, laborious approaches. The goal of this study was the design and validation of a disposable microfluidic platform capable of selectively capturing and enumerating EPCs directly from human whole blood in healthy and diseased subjects, eliminating sample preprocessing. We then applied the "EPC capture chip" clinically and determined EPC numbers in blood from patients with pulmonary arterial hypertension (PAH). Blood was collected in tubes and injected into polymeric microfluidic chips containing microcolumns pre-coated with anti-CD34 antibody. Captured cells were immunofluorescently stained for the expression of stem and endothelial antigens, identified and counted. The EPC capture chip was validated with conventional flow cytometry counts (r = 0.83). The inter- and intra-day reliability of the microfluidic devices was confirmed at different time points in triplicates over 1-5 months. In a cohort of 43 patients with three forms of PAH (idiopathic/heritable, drug-induced, and connective tissue disease), EPC numbers are ≈50% lower in PAH subjects vs. matched controls and inversely related to two potential disease modifiers: body mass index and postmenopausal status. The EPC capture chip (5 × 30 × 0.05 mm(3)) requires only 200 μL of human blood and has the strong potential to serve as a rapid bedside test for the screening and monitoring of patients with PAH and other proliferative cardiovascular, pulmonary, malignant, and neurodegenerative diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.