ArticleBiology of reproduction2011
Expression of Tubb3, a beta-tubulin isotype, is regulated by androgens in mouse and rat Sertoli cells.
Article in Biology of reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- The dynamics of ciliogenesis in prepubertal mouse meiosis reveals new clues about testis maturation.Frontiers in cell and developmental biology · 2026Article
- Early female germline development inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- PTBP1 mediates Sertoli cell actin cytoskeleton organization by regulating alternative splicing of actin regulators.Nucleic acids research · 2024Article
- PTBP1 mediates Sertoli cell actin cytoskeleton organization by regulating alternative splicing of actin regulators.bioRxiv : the preprint server for biology · 2024Article
- Hormone Regulation in Testicular Development and Function.International journal of molecular sciences · 2024Review
- TUBB3 and KIF21A in neurodevelopment and disease.Frontiers in neuroscience · 2023Review
- Concordant Androgen-Regulated Expression of Divergent Rhox5 Promoters in Sertoli Cells.Endocrinology · 2022Article
- βIII-Tubulin Gene Regulation in Health and Disease.Frontiers in cell and developmental biology · 2022Review
- What Does Androgen Receptor Signaling Pathway in Sertoli Cells During Normal Spermatogenesis Tell Us?Frontiers in endocrinology · 2022Review
- Exosomal TUBB3 mRNA expression of metastatic castration-resistant prostate cancer patients: Association with patient outcome under abiraterone.Cancer medicine · 2021Article
- Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition.Cell reports · 2021Article
- Response to: X-linked miR-506 family miRNAs promote FMRP expression in mouse spermatogonia.EMBO reports · 2020Article
- Microtubular Dysfunction and Male Infertility.The world journal of men's health · 2020Review
- Review
- Molecular and genetic characterization of partial masculinization in embryonic ovaries grafted into male nude mice.PloS one · 2019Article
- AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice.Scientific reports · 2018Article
- Androgen Receptor Regulates the Growth of Neuroblastoma CellsFrontiers in neuroscience · 2017Article
- A tubulin alpha 8 mouse knockout model indicates a likely role in spermatogenesis but not in brain development.PloS one · 2017Article
- Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor.International journal of molecular sciences · 2015Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our previous analysis of Sertoli cell androgen receptor (AR) knockout (SCARKO) mice revealed that several cytoskeletal components are a potential target of androgen action. Here, we found that one of these components, the beta-tubulin isotype Tubb3, is differentially regulated in testes from SCARKO mice (relative to littermate controls) from Postnatal Day 10 to adulthood. The Tubb3 gene is unique in this respect, as at Day 10, no other beta-tubulin genes are significantly regulated by AR. We further characterized androgen regulation of Tubb3 in vivo and in vitro and demonstrated that it is a conserved feature in both mice and rats. To investigate whether androgens directly regulate Tubb3 expression, we screened for androgen response elements (AREs) in the Tubb3 gene. In silico analysis revealed the presence of four ARE motifs in Tubb3 intron 1, two of which bind to AR in vitro. Mutation of one of these (ARE1) strongly reduced androgen-dependent reporter gene expression. These results, coupled with the finding that the AR binds to the Tubb3 ARE region in vivo, suggest that Tubb3 is a direct target of AR. Our data strengthen the contention that androgens exert their effects on spermatogenesis, in part, through modulation of the Sertoli cell cytoskeleton. Androgen regulation of beta-tubulin has also been described in neurons, fortifying the already known similarity in microtubule organization in Sertoli cell processes and neurons, the only other cell type in which Tubb3 is known to be expressed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.