ArticleThe Journal of biological chemistry2011
Structural and functional studies on the N-terminal domain of the Shigella type III secretion protein MxiG.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- A flexible peptide linking the periplasmic and cytoplasmic domains of MxiG controls type III secretion signaling and stable sorting platform assembly inFrontiers in cellular and infection microbiology · 2025Article
- Interbacterial competition mediated by the type VIIb secretion system.Microbiology (Reading, England) · 2023Review
- TheMicroorganisms · 2021Review
- The Structures of SctK and SctD from Pseudomonas aeruginosa Reveal the Interface of the Type III Secretion System Basal Body and Sorting Platform.Journal of molecular biology · 2020Article
- The cytoplasmic domain of MxiG interacts with MxiK and directs assembly of the sorting platform in theThe Journal of biological chemistry · 2019Article
- The cytoplasmic portion of the T3SS inner membrane ring components sort into distinct families based on biophysical properties.Biochimica et biophysica acta. Proteins and proteomics · 2019Article
- A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome.Nature communications · 2017Article
- Article
- The Structure and Function of Type III Secretion Systems.Microbiology spectrum · 2016Review
- Composition, formation, and regulation of the cytosolic c-ring, a dynamic component of the type III secretion injectisome.PLoS biology · 2015Article
- Building a secreting nanomachine: a structural overview of the T3SS.Current opinion in structural biology · 2014Review
- Structure and biophysics of type III secretion in bacteria.Biochemistry · 2013Review
- The architecture of EssB, an integral membrane component of the type VII secretion system.Structure (London, England : 1993) · 2013Article
- A refined model of the prototypical Salmonella SPI-1 T3SS basal body reveals the molecular basis for its assembly.PLoS pathogens · 2013Article
- Structure and interactions of the cytoplasmic domain of the Yersinia type III secretion protein YscD.Journal of bacteriology · 2012Article
- Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2012Review
- The blueprint of the type-3 injectisome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Structure of the cytoplasmic domain of Yersinia pestis YscD, an essential component of the type III secretion system.Acta crystallographica. Section D, Biological crystallography · 2012Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
MxiG is a single-pass membrane protein that oligomerizes within the inner membrane ring of the Shigella flexneri type III secretion system (T3SS). The MxiG N-terminal domain (MxiG-N) is the predominant cytoplasmic structure; however, its role in T3SS assembly and secretion is largely uncharacterized. We have determined the solution structure of MxiG-N residues 6-112 (MxiG-N(6-112)), representing the first published structure of this T3SS domain. The structure shows strong structural homology to forkhead-associated (FHA) domains. Canonically, these cell-signaling modules bind phosphothreonine (Thr(P)) via highly conserved residues. However, the putative phosphate-binding pocket of MxiG-N(6-112) does not align with other FHA domain structures or interact with Thr(P). Furthermore, mutagenesis of potential phosphate-binding residues has no effect on S. flexneri T3SS assembly and function. Therefore, MxiG-N has a novel function for an FHA domain. Positioning of MxiG-N(6-112) within the EM density of the S. flexneri needle complex gives insight into the ambiguous stoichiometry of the T3SS, supporting models with 24 MxiG subunits in the inner membrane ring.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.