ArticleACS chemical neuroscience2011
Two-photon microscopy for chemical neuroscience.
Article in ACS chemical neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Thioflavin-T derivatives with controlled AIE and ACQ properties as potential one-photon and two-photon amyloid markers.Scientific reports · 2026Article
- Geometric principles of dendritic integration of excitation and inhibition in cortical neurons.Science advances · 2026Article
- Review
- Dual Infrared 2-Photon Microscopy Achieves Minimal Background Deep Tissue Imaging in Brain and Plant Tissues.Advanced functional materials · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy.Neurophotonics · 2023Article
- Advances in the Electrophysiological Recordings of Long-Term Potentiation.International journal of molecular sciences · 2023Review
- Intracellular photoswitchable neuropharmacology driven by luminescence from upconverting nanoparticles.Chemical communications (Cambridge, England) · 2020Article
- Dendrimer Conjugation Enables Multiphoton Chemical Neurophysiology Studies with an Extended π-Electron Caging Chromophore.Angewandte Chemie (International ed. in English) · 2019Article
- Reversible silencing of endogenous receptors in intact brain tissue using 2-photon pharmacology.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Caged Proline in Photoinitiated Organocatalysis.The Journal of organic chemistry · 2019Article
- Cobalt oxyhydroxide modified with poly-β-cyclodextrin and a cyanine dye as a nanoplatform for two-photon imaging of ascorbic acid in living cells and tissue.Mikrochimica acta · 2019Article
- Photochemical generation of the 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) radical from caged nitroxides by near-infrared two-photon irradiation and its cytocidal effect on lung cancer cells.Beilstein journal of organic chemistry · 2019Article
- Improved Synthesis of Caged Glutamate and Caging Each Functional Group.ACS chemical neuroscience · 2018Article
- Two-Photon Uncaging of Glutamate.Frontiers in synaptic neuroscience · 2018Review
- Intracellular Uncaging of cGMP with Blue Light.ACS chemical neuroscience · 2017Article
- A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System.Scientific reports · 2017Article
- Cloaked Caged Compounds: Chemical Probes for Two-Photon Optoneurobiology.Angewandte Chemie (International ed. in English) · 2017Article
- Synthesis of Near-Infrared Fluorescent Two-Photon-Absorbing Fluorenyl Benzothiadiazole and Benzoselenadiazole Derivatives.ACS omega · 2016Article
- Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Microscopes using non-linear excitation of chromophores with pulsed near-IR light can generate highly localized foci of molecules in the electronic singlet state that are concentrated in volumes of less than one femtoliter. The three-dimensional confinement of excitation arises from the simultaneous absorption of two IR photons of approximately half the energy required for linear excitation. Two-photon microscopy is especially useful for two types of interrogation of neural processes. First, uncaging of signaling molecules such as glutamate, as stimulation is so refined it can be used to mimic normal unitary synaptic levels. In addition, uncaging allows complete control of the timing and position of stimulation, so the two-photon light beam provides the chemical neuroscientist with an "optical conductor's baton" which can command synaptic activity at will. A second powerful feature of two-photon microscopy is that when used for fluorescence imaging it enables the visualization of cellular structure and function in living animals at depths far beyond that possible with normal confocal microscopes. In this review I provide a survey of the many important applications of two-photon microscopy in these two fields of neuroscience, and suggest some areas for future technical development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.