ArticlePloS one2011
Transformation of human mesenchymal cells and skin fibroblasts into hematopoietic cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Dopaminergic progenitors generated by small molecule approach survived, integrated, and promoted functional recovery in (6-OHDA) mouse model of Parkinson's disease.Journal of the neurological sciences · 2024Article
- Reprogramming of fibroblast cells to totipotent state by DNA demethylation.Scientific reports · 2023Article
- All roads lead to Rome: the many ways to pluripotency.Journal of assisted reproduction and genetics · 2020Review
- Stem cells out of the bag: characterization ofFuture science OA · 2020Article
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Modulating the distribution and fate of exogenously delivered MSCs to enhance therapeutic potential: knowns and unknowns.Intensive care medicine experimental · 2019Review
- STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells.Oncotarget · 2018Article
- Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018Article
- Erase and Rewind: Epigenetic Conversion of Cell Fate.Stem cell reviews and reports · 2016Review
- HOX transcription factors are potential targets and markers in malignant mesothelioma.BMC cancer · 2016Article
- The quest for an effective and safe personalized cell therapy using epigenetic tools.Clinical epigenetics · 2016Review
- Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4.Stem cells international · 2015Article
- Functional comparison of induced pluripotent stem cell- and blood-derived GPIIbIIIa deficient platelets.PloS one · 2015Article
- Mir-218 contributes to the transformation of 5-Aza/GF induced umbilical cord mesenchymal stem cells into hematopoietic cells through the MITF pathway.Molecular biology reports · 2014Article
- The significance of spliceosome mutations in chronic lymphocytic leukemia.Leukemia & lymphoma · 2013Review
- Dopaminergic-like cells from epigenetically reprogrammed mesenchymal stem cells.Journal of cellular and molecular medicine · 2012Article
- Do the Fibroblasts Contained in Early Passage MSC Population Adversely Affect the Characteristics of Stem Cell Population Obtained from Human Placenta?International journal of stem cells · 2012Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Patients with prolonged myelosuppression require frequent platelet and occasional granulocyte transfusions. Multi-donor transfusions induce alloimmunization, thereby increasing morbidity and mortality. Therefore, an autologous or HLA-matched allogeneic source of platelets and granulocytes is needed. To determine whether nonhematopoietic cells can be reprogrammed into hematopoietic cells, human mesenchymal stromal cells (MSCs) and skin fibroblasts were incubated with the demethylating agent 5-azacytidine (Aza) and the growth factors (GF) granulocyte-macrophage colony-stimulating factor and stem cell factor. This treatment transformed MSCs to round, non-adherent cells expressing T-, B-, myeloid-, or stem/progenitor-cell markers. The transformed cells engrafted as hematopoietic cells in bone marrow of immunodeficient mice. DNA methylation and mRNA array analysis suggested that Aza and GF treatment demethylated and activated HOXB genes. Indeed, transfection of MSCs or skin fibroblasts with HOXB4, HOXB5, and HOXB2 genes transformed them into hematopoietic cells. Further studies are needed to determine whether transformed MSCs or skin fibroblasts are suitable for therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.