ArticlePloS one2011
An information theoretic, microfluidic-based single cell analysis permits identification of subpopulations among putatively homogeneous stem cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed.
- Endothelial-specific CXCL12 regulates neovascularization during tissue repair and tumor progression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- A top-down measure of gene-to-gene coordination for analyzing cell-to-cell variability.Scientific reports · 2021Article
- Characterization of Diabetic and Non-Diabetic Foot Ulcers Using Single-Cell RNA-Sequencing.Micromachines · 2020Article
- Small molecule inhibition of dipeptidyl peptidase-4 enhances bone marrow progenitor cell function and angiogenesis in diabetic wounds.Translational research : the journal of laboratory and clinical medicine · 2019Article
- Single-Cell Transcriptomics of Human Mesenchymal Stem Cells Reveal Age-Related Cellular Subpopulation Depletion and Impaired Regenerative Function.Stem cells (Dayton, Ohio) · 2019Article
- Spatial Analysis of Single Fiber Cells of the Developing Ocular Lens Reveals Regulated Heterogeneity of Gene Expression.iScience · 2018Article
- Single-Cell Gene Expression Analysis and Evaluation of the Therapeutic Function of Murine Adipose-Derived Stromal Cells (ASCs) from the Subcutaneous and Visceral Compartment.Stem cells international · 2018Article
- Delivery of monocyte lineage cells in a biomimetic scaffold enhances tissue repair.JCI insight · 2017Article
- The Role of Focal Adhesion Kinase in Keratinocyte Fibrogenic Gene Expression.International journal of molecular sciences · 2017Article
- Isolation of CD248-expressing stromal vascular fraction for targeted improvement of wound healing.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2017Article
- Regenerative Medicine: Charting a New Course in Wound Healing.Advances in wound care · 2016Review
- Microfluidic single-cell transcriptional analysis rationally identifies novel surface marker profiles to enhance cell-based therapies.Nature communications · 2016Article
- Adipose-Derived Stem Cell-Seeded Hydrogels Increase Endogenous Progenitor Cell Recruitment and Neovascularization in Wounds.Tissue engineering. Part A · 2016Article
- High-Resolution Microfluidic Single-Cell Transcriptional Profiling Reveals Clinically Relevant Subtypes among Human Stem Cell Populations Commonly Utilized in Cell-Based Therapies.Frontiers in neurology · 2016Review
- Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis.Microarrays (Basel, Switzerland) · 2015Article
- Progenitor cell dysfunctions underlie some diabetic complications.The American journal of pathology · 2015Review
- Skin fibrosis. Identification and isolation of a dermal lineage with intrinsic fibrogenic potential.Science (New York, N.Y.) · 2015Article
- Studies in fat grafting: Part IV. Adipose-derived stromal cell gene expression in cell-assisted lipotransfer.Plastic and reconstructive surgery · 2015Article
- Isolation and enrichment of human adipose-derived stromal cells for enhanced osteogenesis.Journal of visualized experiments : JoVE · 2015Article
- Mesenchymal Stem Cells and Cutaneous Wound Healing: Current Evidence and Future Potential.Stem cells international · 2015Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
An incomplete understanding of the nature of heterogeneity within stem cell populations remains a major impediment to the development of clinically effective cell-based therapies. Transcriptional events within a single cell are inherently stochastic and can produce tremendous variability, even among genetically identical cells. It remains unclear how mammalian cellular systems overcome this intrinsic noisiness of gene expression to produce consequential variations in function, and what impact this has on the biologic and clinical relevance of highly 'purified' cell subgroups. To address these questions, we have developed a novel method combining microfluidic-based single cell analysis and information theory to characterize and predict transcriptional programs across hundreds of individual cells. Using this technique, we demonstrate that multiple subpopulations exist within a well-studied and putatively homogeneous stem cell population, murine long-term hematopoietic stem cells (LT-HSCs). These subgroups are defined by nonrandom patterns that are distinguishable from noise and are consistent with known functional properties of these cells. We anticipate that this analytic framework can also be applied to other cell types to elucidate the relationship between transcriptional and phenotypic variation.
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