ArticlePloS one2011
Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed, 158 citations in OpenAlex.
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- An atlas of small non-coding RNAs in human preimplantation development.Nature communications · 2024Article
- Interconnected Codons: Unravelling the Epigenetic Significance of Flanking Sequences in CpG Dyads.Journal of molecular evolution · 2024Article
- Chromatin Modifier EP400 Regulates Oocyte Quality and Zygotic Genome Activation in Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Omics Views of Mechanisms for Cell Fate Determination in Early Mammalian Development.Genomics, proteomics & bioinformatics · 2023Review
- N6-methyladenosine (mProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Expression and function of PDGF-C in development and stem cells.Open biology · 2021Review
- Transcription factor OTX2 silences the expression of cleavage embryo genes and transposable elements.The Journal of reproduction and development · 2021Article
- Feasibility of predicting allele specific expression from DNA sequencing using machine learning.Scientific reports · 2021Article
- All models are wrong, but some are useful: Establishing standards for stem cell-based embryo models.Stem cell reports · 2021Review
- Suggestions on cleavage embryo and blastocyst vitrification/transfer based on expression profile of ACE2 and TMPRSS2 in current COVID-19 pandemic.Molecular reproduction and development · 2021Article
- Single-cell Sequencing in the Field of Stem Cells.Current genomics · 2020Review
- BIRD: identifying cell doublets via biallelic expression from single cells.Bioinformatics (Oxford, England) · 2020Article
- Notch signaling in mouse blastocyst development and hatching.BMC developmental biology · 2020Article
- Rybp orchestrates spermatogenesis via regulating meiosis and sperm motility in mice.Cell cycle (Georgetown, Tex.) · 2020Article
- A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs.International journal of molecular sciences · 2020Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
Abstract
Stochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis of single blastomeres of mouse embryos, which revealed significant changes in the transcriptome. Importantly, over half of the transcripts with detectable genetic polymorphisms exhibit ASE, most notably, individual blastomeres from the same two-cell embryo show similar pattern of ASE. However, about 6% of them exhibit stochastic expression, indicated by altered expression ratio between the two alleles. Thus, we demonstrate that ASE is both deterministic and stochastic in early blastomeres. Furthermore, we also found that 1,718 genes express two isoforms with different lengths of 3'UTRs, with the shorter one on average 5-6 times more abundant in early blastomeres compared to the transcripts in epiblast cells, suggesting that microRNA mediated regulation of gene expression acquires increasing importance as development progresses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.