ArticlePloS one2011
High-level fluorescence labeling of gram-positive pathogens.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
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- Spatial population dynamics of bacterial colonies with social antibiotic resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Intraspecies interactions ofbioRxiv : the preprint server for biology · 2023Article
- Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms.Biomedicines · 2023Article
- pBFK, a new thermosensitive shuttle vector forHeliyon · 2023Article
- Efficient fluorescence-based localization technique for real-time tracking endophytes route in host-plants colonization.Plant direct · 2022Article
- The Role of SilX in Bacteriocin Production ofFrontiers in microbiology · 2022Article
- Group B Streptococcus CAMP Factor Does Not Contribute to Interactions with the Vaginal Epithelium and Is Dispensable for Vaginal Colonization in Mice.Microbiology spectrum · 2021Article
- CRIg on liver macrophages clears pathobionts and protects against alcoholic liver disease.Nature communications · 2021Article
- Spatial segregation and cooperation in radially expanding microbial colonies under antibiotic stress.The ISME journal · 2021Article
- SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression.Nature protocols · 2021Article
- Mucin O-glycans suppress quorum-sensing pathways and genetic transformation in Streptococcus mutans.Nature microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Fluorescence labeling of bacterial pathogens has a broad range of interesting applications including the observation of living bacteria within host cells. We constructed a novel vector based on the E. coli streptococcal shuttle plasmid pAT28 that can propagate in numerous bacterial species from different genera. The plasmid harbors a promoterless copy of the green fluorescent variant gene egfp under the control of the CAMP-factor gene (cfb) promoter of Streptococcus agalactiae and was designated pBSU101. Upon transfer of the plasmid into streptococci, the bacteria show a distinct and easily detectable fluorescence using a standard fluorescence microscope and quantification by FACS-analysis demonstrated values that were 10-50 times increased over the respective controls. To assess the suitability of the construct for high efficiency fluorescence labeling in different gram-positive pathogens, numerous species were transformed. We successfully labeled Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus dysgalactiae subsp. equisimilis, Enterococcus faecalis, Enterococcus faecium, Streptococcus mutans, Streptococcus anginosus and Staphylococcus aureus strains utilizing the EGFP reporter plasmid pBSU101. In all of these species the presence of the cfb promoter construct resulted in high-level EGFP expression that could be further increased by growing the streptococcal and enterococcal cultures under high oxygen conditions through continuous aeration.
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