ArticleJournal of visualized experiments : JoVE2011
A system for culturing iris pigment epithelial cells to study lens regeneration in newt.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- A Review of Histocytological Events and Molecular Mechanisms Involved in Intestine Regeneration in Holothurians.Biology · 2022Review
- In Vivo Imaging of Newt Lens Regeneration: Novel Insights Into the Regeneration Process.Translational vision science & technology · 2021Article
- Insights into regeneration tool box: An animal model approach.Developmental biology · 2019Review
- Molecular signatures that correlate with induction of lens regeneration in newts: lessons from proteomic analysis.Human genomics · 2014Article
- Plasticity for axolotl lens regeneration is associated with age-related changes in gene expression.Regeneration (Oxford, England) · 2014Article
- Intrinsic lens forming potential of mouse lens epithelial versus newt iris pigment epithelial cells in three-dimensional culture.Tissue engineering. Part C, Methods · 2014Article
- Exogenous Oct-4 inhibits lens transdifferentiation in the newt Notophthalmus viridescens.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Salamanders like newt and axolotl possess the ability to regenerate many of its lost body parts such as limbs, the tail with spinal cord, eye, brain, heart, the jaw¹. Specifically, newts are unique for its lens regeneration capability. Upon lens removal, IPE cells of the dorsal iris transdifferentiate to lens cells and eventually form a new lens in about a month²(,)³ . This property of regeneration is never exhibited by the ventral iris cells. The regeneration potential of the iris cells can be studied by making transplants of the in vitro cultured IPE cells. For the culture, the dorsal and ventral iris cells are first isolated from the eye and cultured separately for a time period of 2 weeks (Figure 1). These cultured cells are reaggregated and implanted back to the newt eye. Past studies have shown that the dorsal reaggregate maintains its lens forming capacity whereas the ventral aggregate does not form a lens, recapitulating, thus the in vivo process (Figure 2)⁴(,)⁵. This system of determining regeneration potential of dorsal and ventral iris cells is very useful in studying the role of genes and proteins involved in lens regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.