ArticleProceedings of the National Academy of Sciences of the United States of America2011
Single-molecule investigations of the stringent response machinery in living bacterial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 148 papers.
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Who cites it
148 citing papers in PubMed, 280 citations in OpenAlex.
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- Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-codedRSC chemical biology · 2026Article
- The opposite effects of stringent response on phage infection ofmicroLife · 2026Article
- Robust Quantification of Live-Cell Single-Molecule Tracking Data for Fluorophores with Different Photophysical Properties.The journal of physical chemistry. B · 2024Article
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Visualizing the invisible: novel approaches to visualizing bacterial proteins and host-pathogen interactions.Frontiers in bioengineering and biotechnology · 2024Review
- Simulation-based Reconstructed Diffusion unveils the effect of aging on protein diffusion in Escherichia coli.PLoS computational biology · 2023Article
- CcpA and CodY Regulate CRISPR-Cas System ofMicrobiology spectrum · 2023Article
- Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- A model for ribosome translocation based on the alternated displacement of its subunits.European biophysics journal : EBJ · 2023Article
- Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization.Nature communications · 2023Article
- Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes.Communications biology · 2023Article
- Conformation of von Willebrand factor in shear flow revealed with stroboscopic single-molecule imaging.Blood · 2022Article
- Dependence of diffusion ineLife · 2022Article
- The Importance of Microorganisms for Sustainable Agriculture-A Review.Metabolites · 2022Review
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- Protein diffusion inScience advances · 2022Article
- Transcriptomic profiling of Escherichia coli K-12 in response to a compendium of stressors.Scientific reports · 2022Article
- What Is Motion? Recent Advances in the Study of Molecular Movement Patterns of the Peptidoglycan Synthesis Machines.Journal of bacteriology · 2022Review
88 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RelA-mediated stringent response is at the heart of bacterial adaptation to starvation and stress, playing a major role in the bacterial cell cycle and virulence. RelA integrates several environmental cues and synthesizes the alarmone ppGpp, which globally reprograms transcription, translation, and replication. We have developed and implemented novel single-molecule tracking methodology to characterize the intracellular catalytic cycle of RelA. Our single-molecule experiments show that RelA is on the ribosome under nonstarved conditions and that the individual enzyme molecule stays off the ribosome for an extended period of time after activation. This suggests that the catalytically active part of the RelA cycle is performed off, rather than on, the ribosome, and that rebinding to the ribosome is not necessary to trigger each ppGpp synthesis event. Furthermore, we find fast activation of RelA in response to heat stress followed by RelA rapidly being reset to its inactive state, which makes the system sensitive to new environmental cues and hints at an underlying excitable response mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.