ArticleProceedings of the National Academy of Sciences of the United States of America1990
Introduction of nerve growth factor (NGF) receptors into a medulloblastoma cell line results in expression of high- and low-affinity NGF receptors but not NGF-mediated differentiation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 71 citations in OpenAlex.
- Primitive neuroectodermal tumors of the central nervous system.Brain pathology (Zurich, Switzerland) · 1997Review
- Nerve growth factor induces apoptosis in human medulloblastoma cell lines that express TrkA receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Growth factor receptors and medulloblastoma.Journal of neuro-oncology · 1996Review
- The neurotrophin receptor, gp75, forms a complex with the receptor tyrosine kinase TrkA.The Journal of cell biology · 1996Article
- Neurotrophin and neurotrophin receptor proteins in medulloblastomas and other primitive neuroectodermal tumors of the pediatric central nervous system.The American journal of pathology · 1996Article
- Expression of the neurotrophin receptor TrkC is linked to a favorable outcome in medulloblastoma.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Circular dichroism and crosslinking studies of the interaction between four neurotrophins and the extracellular domain of the low-affinity neurotrophin receptor.Protein science : a publication of the Protein Society · 1994Article
- Ectopic trkA expression mediates a NGF survival response in NGF-independent sensory neurons but not in parasympathetic neurons.The Journal of cell biology · 1993Article
- Expression of trkA cDNA in neuroblastomas mediates differentiation in vitro and in vivo.Molecular and cellular biology · 1993Article
- Chimeric tumor necrosis factor-TrkA receptors reveal that ligand-dependent activation of the TrkA tyrosine kinase is sufficient for differentiation and survival of PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Differential expression of nerve growth factor receptors leads to altered binding affinity and neurotrophin responsiveness.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Monoclonal antibodies to a rat nestin fusion protein recognize a 220-kDa polypeptide in subsets of fetal and adult human central nervous system neurons and in primitive neuroectodermal tumor cells.The American journal of pathology · 1993Article
- Spectroscopic and chemical studies of the interaction between nerve growth factor (NGF) and the extracellular domain of the low affinity NGF receptor.Protein science : a publication of the Protein Society · 1992Article
- Concentration-dependent regulation of neuronal gene expression by nerve growth factor.The Journal of cell biology · 1992Article
- The nerve growth factor receptor: a multicomponent system that mediates the actions of the neurotrophin family of proteins.Molecular and cellular biochemistry · 1992Review
- Identification of tyrosine kinase Trk as a nerve growth factor receptor.Cell regulation · 1991Review
- The low-affinity p75 nerve growth factor (NGF) receptor mediates NGF-induced tyrosine phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Article
- Nerve growth factor receptor expression in peripheral and central neuroectodermal tumors, other pediatric brain tumors, and during development of the adrenal gland.The American journal of pathology · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the cloned human nerve growth factor receptor (NGFR) cDNA in cell lines can generate both high- and low-affinity binding sites. Since the inability to respond appropriately to differentiation factors such as NGF may contribute to determining the malignant phenotype of neuroblastomas, we sought to determine whether the same is true of medulloblastomas. To generate a human central nervous system neuronal cell line that would respond to NGF, we infected the medulloblastoma cell line D283 MED with a defective retrovirus carrying the cDNA coding for the human NGFR. The resultant cells (MED-NGFR) expressed abundant low- and high-affinity NGFRs, and NGF treatment induced a rapid transient increase of c-fos mRNA in the NGFR-expressing cells but not in the parent line or in cells infected with virus lacking the cDNA insert. However, the MED-NGFR cells did not internalize the NGFR at high efficiency, nor did they differentiate in response to NGF. Three important conclusions emerge from this study: (i) internalization of NGFRs is not necessary for some early rapid transcriptional effects of NGF; (ii) an unknown factor(s) that cooperates with the cloned NGFR in allowing high-affinity NGF binding is found in a primitive central nervous system cell line; and (iii) NGFRs introduced into and expressed by D283 MED (i.e., MED-NGFR) cells are partially functional but are unable to induce differentiation in these primitive neuron-like tumor cells, implying that high-efficiency receptor-mediated endocytosis of NGF and its receptor may be a necessary step in the cascade of events leading to NGF-mediated differentiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.