ArticleProceedings of the National Academy of Sciences of the United States of America1990
Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
73 citing papers in PubMed, 207 citations in OpenAlex.
- Regulation of iron ion homeostasis inmBio · 2026Review
- Labile iron pool dynamics do not drive ferroptosis in colorectal cancer cells.The Journal of biological chemistry · 2026Article
- Mapping the genetic landscape of iron metabolism uncovers the SETD2 methyltransferase as a modulator of iron flux.Science advances · 2025Article
- Labile iron pool dynamics do not drive ferroptosis potentiation in colorectal cancer cells.bioRxiv : the preprint server for biology · 2025Article
- Tracking hemopexin intracellularly and defining hemopexin protein "interactomes" in human immune and liver cell models.Frontiers in physiology · 2025Article
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- Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis.Experimental eye research · 2022Article
- Host and microbiota derived extracellular vesicles: Crucial players in iron homeostasis.Frontiers in medicine · 2022Review
- Iron Regulatory Protein 2 Exerts its Oncogenic Activities by Suppressing TAp63 Expression.Molecular cancer research : MCR · 2020Article
- Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of CaCells · 2020Article
- Ferroportin-mediated iron export from vascular endothelial cells in retina and brain.Experimental eye research · 2019Article
- Liver-Specific, but Not Retina-Specific, Hepcidin Knockout Causes Retinal Iron Accumulation and Degeneration.The American journal of pathology · 2019Article
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- The indispensable role of mammalian iron sulfur proteins in function and regulation of multiple diverse metabolic pathways.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2019Review
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- Dysregulation of Neuronal Iron Homeostasis as an Alternative Unifying Effect of Mutations Causing Familial Alzheimer's Disease.Frontiers in neuroscience · 2018Article
- Biogenesis and functions of mammalian iron-sulfur proteins in the regulation of iron homeostasis and pivotal metabolic pathways.The Journal of biological chemistry · 2017Review
- Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.Investigative ophthalmology & visual science · 2017Article
- Ferredoxin reductase is critical for p53-dependent tumor suppression via iron regulatory protein 2.Genes & development · 2017Article
- Metabolic Enzymes Enjoying New Partnerships as RNA-Binding Proteins.Trends in endocrinology and metabolism: TEM · 2015Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Iron-responsive elements (IREs) are stemloop structures found in the mRNAs encoding ferritin and the transferrin receptor. These elements participate in the iron-induced regulation of the translation of ferritin and the stability of the transferrin receptor mRNA. Regulation in both instances is mediated by binding of a cytosolic protein to the IREs. High-affinity binding is seen when cells are starved of iron and results in repression of ferritin translation and inhibition of transferrin receptor mRNA degradation. The IRE-binding protein (IRE-BP) has been identified as an approximately 90-kDa protein that has been purified by both affinity and conventional chromatography. In this report we use RNA affinity chromatography and two-dimensional gel electrophoresis to isolate the IRE-BP for protein sequencing. A degenerate oligonucleotide probe derived from a single peptide sequence was used to isolate a cDNA clone that encodes a protein containing 13 other sequenced peptides obtained from the IRE-BP. Consistent with previous characterization of the IRE-BP, the cDNA encodes a protein of 87 kDa with a slightly acidic pI, and the corresponding mRNA of approximately 3.6 kilobases is found in a variety of cell types. The encoded protein contains a nucleotide-binding consensus sequence and regions of cysteine and histidine clusters. This mRNA is encoded by a single gene on human chromosome 9, a finding consistent with previous localization by functional mapping. The protein contains no previously defined consensus motifs for either RNA or DNA binding. The simultaneous cloning of a different, but highly homologous, cDNA suggests that the IRE-BP is a member of a distinct gene family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.